次氯酸钠对扁浒苔叶绿素荧光特性及光合速率的影响

以江苏如东紫菜养殖海区的绿藻群落构成种扁浒苔(Ulva compressa)为研究对象,通过叶绿素提取法、叶绿素荧光技术及液相氧电极研究了不同浓度次氯酸钠溶液对其叶绿素组成、荧光特性和光合速率的作用情况。结果表明:扁浒苔叶绿素含量和荧光特性随着NaClO浓度的升高均呈现逐渐递减的趋势,经10.0 mmol/L的NaClO溶液处理后,藻体叶绿素a和叶绿素b的含量分别为0.030 mg/g,0.039 mg/g;0~6.00 mmol/L的NaClO对扁浒苔叶绿素荧光参数Fv/Fm有显著影响;10.0~100.0μmol/L次氯酸钠在3 min就能使扁浒苔的光合放氧速率大幅下降,其中100μmol/L组从对照组的1 315 nmol O2/(min·g)降至-472 nmol O2/(min·g)。根据实验结果和实际海区验证,4.0mmol/L的NaClO可以作为清除紫菜养殖海区绿藻的理想试剂。

Effects of sodium hypochlorite on chlorophyll fluorescence parameters and photosynthetic rate of Ulva compressa

This paper used Ulva compressa which is the composing species of green algae communities in Rudong seaweed farming sea area for investigation. Experiments studied the effects of sodium hypochlorite on chlorophyll composition, fluorescence characteristics and photosynthetic rate of U. compressa by chlorophyll extract, chlorophyll fluorescence technology and liquid oxygen electrode. The results showed that with the NaC10 concentration increasing, the chlorophyll content and fluorescence characteristics of U. compressa were gradually decreasing. After 10.0 mmol/L NaC10 treatment, Chl. a and Chl. b respectively reduced to 0. 030 mg/g and 0. 039 rag/g; 0. 00 - 6. 00 mmol/L NaC10 have significant influence on the fluorescence characteristics of the algae; 10.0 - 100 μmol/L NaC10 can make the photosynthetic rate of U. compressa decline significantly in 3 min, in which 100μmol/L group declined from the control group of 1 315 nmol 02/ （ min·g ） to - 472 nmol 02/（ min· g ）. According to the experiments and verification in aquaculture area, 4.0 mmol/L NaC10 could be regarded as an ideal reagent in green algae removing.