斑马鱼下颌再生中神经与肌肉的关系

本研究旨在揭示斑马鱼下颌再生中神经和肌肉的关系。通过HE染色技术、免疫荧光组织化学等实验技术分别观察斑马鱼下颌神经和肌肉的再生过程,考察神经和肌肉的关系。研究结果显示,截面附近的神经纤维在剪切后5 h降解,剪切后2 d时完全消失。剪切后3 d,整个芽基所在位置已出现新生的神经纤维。剪切5 d时,芽基上布满大量的神经纤维。肌纤维在剪切后12 h已发生断裂,剪切后5 d形成芽基细胞并分化成各种前体细胞如肌肉前体细胞,剪切后9 d出现新生的肌细胞,剪切后14 d新生的肌细胞与新生的神经纤维建立神经肌肉接头联系,30 d后成为成熟的肌肉组织。结果表明:如果斑马鱼1/3的下颌被人为切除,下颌肌肉可以实现功能性再生,同时神经极可能在肌肉再生中发挥作用。但是如果切除部分超过1/3,则下颌无法再生复原,其原因有待进一步研究。

The relationships between nerve and muscle during lower jaw regeneration in adult zebra fish, Danio rerio

This research investigated the relationships between nerve and muscle in regeneration of lower jaw muscle of the adult zebra fish, Danio rerio. We observed and analyzed the regeneration process of the lower jaw muscles by using histological methods via hematoxylin/eosin staining and immunohistochemistry after amputating different proportions of the lower jaw. Our results indicated that after the distal one-third of the lower jaw was amputated, both of the muscle and nerve fibers degenerated rapidly. The nerve fibers began extending over the cutting surface by 2 days-post-amputation (dpa), and they reached the degenerating tissue prior to the blastema formation. The number of nerve fibers increased significantly at 5 dpa, while the blastema was formed. The new muscle fibers arose at 9 dpa, but without forming the neuromuscular junctions. The neuromuscular junctions began to form at approximately 14 dpa. By 30 dpa, the pattern of muscle fibers and neuromuscular junctions recovered to the control level. The results demonstrated that the muscles in zebrafish lower jaw can regenerate functionally. They also suggest that nerve fibers are important for lower jaw regeneration in zebrafish. We also found that if the amputation portion was over 1/3 of the lower jaw, the lower jaw won't be regenerated. The reason of that takes further study.