| 斑节对虾谷氨酰胺合成酶基因的克隆及氨氮胁迫对其时空表达的影响 |
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| 引用本文： | 陈劲松,周发林,江世贵,杨其彬,马振华,邱丽华,傅明骏,李运东,黄建华.斑节对虾谷氨酰胺合成酶基因的克隆及氨氮胁迫对其时空表达的影响[J].上海海洋大学学报,2016,25(4):497-507. |
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| 作者姓名： | 陈劲松 周发林 江世贵 杨其彬 马振华 邱丽华 傅明骏 李运东 黄建华 |
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| 作者单位： | 中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所,中国水产科学研究院 南海水产研究所 |
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| 基金项目： | 虾产业技术体系（CARS-47） |
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| 摘 要： | 根据本实验室构建的斑节对虾(Penaeus monodon)c DNA文库得到的EST序列,利用RACE技术获得了斑节对虾谷氨酰胺合成酶基因(Pm GS)的c DNA全长序列,进行了相关生物信息学分析,在此基础上采用荧光定量的方法研究了Pm GS基因在斑节对虾不同组织、氨氮胁迫过程中差异表达情况。该序列全长1 420bp,开放阅读框(ORF)为1 086 bp,3'非编码区(UTR)为294 bp,包括含有27个碱基的poly(A)尾,5'非编码区(UTR)为40 bp。ORF可编码361个氨基酸,预测分子量为40.423 ku,理论等电点为6.19。序列含有一个谷氨酰胺结合结构域(Gln-synt_C),8个磷酸化位点,2个糖基化位点。斑节对虾和中国明对虾的GS基因的相似性最高,达99%。Pm GS-mRNA在斑节对虾各组织中都有表达,在淋巴组织中表达量最高,其次为鳃组织,在胸神经中的表达量最低。96 h高浓度氨氮胁迫后,Pm GS-mRNA在肝胰腺中的表达水平显著高于对照组,但在鳃中的表达水平显著低于对照组(P0.05)。以上结果暗示,Pm GS在氨氮代谢方面具有重要的作用,可能参与了斑节对虾机体的急性氨氮胁迫应答反应。
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| 关 键 词： | 斑节对虾 谷氨酰胺合成酶 氨氮胁迫 基因克隆 组织表达 |
| 收稿时间： | 2015/9/25 0:00:00 |
| 修稿时间： | 2015/11/22 0:00:00 |
Molecular cloning and the expression analysis of glutamine synthetase (GS) in Penaeus monodon under the condition of ammonia nitrogen stress |
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| CHEN Jinsong,ZHOU Falin,JIANG Shigui,YANG Qibin,MA Zhenhu,QIU Lihu,FU Mingjun,LI Yundong and HUANG Jianhua.Molecular cloning and the expression analysis of glutamine synthetase (GS) in Penaeus monodon under the condition of ammonia nitrogen stress[J].Journal of Shanghai Ocean University,2016,25(4):497-507. |
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| Authors: | CHEN Jinsong ZHOU Falin JIANG Shigui YANG Qibin MA Zhenhu QIU Lihu FU Mingjun LI Yundong and HUANG Jianhua |
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| Institution: | Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China and Key Lab of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China |
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| Abstract: | |
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| Keywords: | Penaeus monodon GS ammonia nitrogen stress gene clone tissues expression |
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