三疣梭子蟹雌激素相关受体基因在蜕皮过程中的表达分析

雌激素相关受体(ERR)在甲壳动物蜕皮过程中可能起着重要的调节作用,但是尚不清楚该基因在三疣梭子蟹不同组织和不同蜕皮阶段的表达模式。本文采用荧光定量PCR(qRT-PCR)技术研究了三疣梭子蟹雌激素相关受体(Pt ERR)基因在不同组织和不同蜕皮阶段的表达情况。结果表明,Pt ERR-mRNA在C期三疣梭子蟹的Y器官和鳃中表达水平最高,其次眼柄和胃中表达水平也相对较高,在3种肌肉组织和三角膜中表达水平较低。在不同蜕皮阶段,13种组织中的Pt ERR-mRNA表达水平均差异显著。Y器官中Pt ERR-mRNA从AB期到E期呈显著上升趋势;胸神经节和眼柄中的Pt ERR-mRNA变化模式均为先上升后下降趋势,大颚器中的Pt ERR-mRNA变化趋势为先下降后上升;大螯肌肉、附肢肌肉和三角膜中的Pt ERR-mRNA表达水平均呈显著升高趋势,而腹部肌肉中Pt ERR-mRNA为先下降后上升趋势;就消化代谢器官而言,胃、肝胰腺和鳃中Pt ERR-mRNA在整个蜕皮周期中均为先升高后降低的趋势,其中D期达最高值;肠道中Pt ERR-mRNA相对表达量在D期最高,C期和E期较低;从AB期至E期,心脏中Pt ERR-mRNA表达水平一直显著增加,每期增加1倍左右。综上,Pt ERR主要在Y器官、鳃、眼柄和胃中表达,可能参与调控蜕皮过程、细胞增殖和能量代谢等。

Expression analysis of PtERR during the molting cycle in Portunus trituberculatus

The swimming crab, Portunus trituberculatus is a commercially important mariculture species in east coast regions of China. Molting is an important physiological process for the growth of P. trituberculatus and estrogen related receptor (ERR) may be involved in the molting regulation. The present study was designed to investigate the expression differences of estrogen related receptor (PtERR) in various tissues of Portunus trituberculatus during molting cycle by quantitative real-time PCR (qRT-PCR). The results showed that, for the tissue-specific expression analysis, the Y-organs and gill had the highest expression levels of PtERR, and the second highest expression levels were found in eyestalk and stomach, while the lower expression levels were found in three kinds of muscle tissues and the triangular membrane at stage C. The expression levels of PtERR varied significantly in any specific tissue at different stages of molting for 13 types of tissues. The expression pattern of PtERR-mRNA showed a significant increasing trend from stage AB to stage E in Y-organ; the pattern of "low-high-low" was found ifor the PtERR-mRNA of thoracic ganglia and eyestalk; the trend of "high-low-high" was found in mandibular organ; PtERR-mRNA had the significantly increasing trend in cheliceral muscle, appendage muscle and triangular membrane. However, the trend of "high-low-high" was found in abdominal muscle. In terms of digestive and metabolic organs, the expression levels of PtERR-mRNA increased firstly and then declined throughout the molting cycle in stomach, hepatopancreas and gill, and the peak value of PtERR-mRNA was detected at stage D; for intestinal tract, the highest expression level of PtERR-mRNA was found at stage D, and lower expression level was detected at stage C and stage E; finally, the expression level of PtERR-mRNA increased significantly in heart from stage AB to stage E, which was around two-fold increase for each stage. In summary, PtERR is mainly expressed in Y-organ, gill, eyestalk and stomach, which may be involved in the regulation of molting process, cell proliferation and energy metabolism.