基于眼柄微结构的克氏原螯虾生长分析

甲壳类的蜕壳生长机制使其能够记录生物学信息的钙化组织周期性消失。一直以来,甲壳类年龄和生长的研究都是一个比较棘手的问题。本文观察和分析了采集于上海浦东新区南汇新城镇河道的克氏原螯虾(Procambarus clarkii)的眼柄微结构,并研究了其眼柄上的生长纹与体长、体质量的关系。结果显示,克氏原螯虾眼柄切片的横截面和纵切面均可观察到其微结构由表层、色素层、钙化层和膜层等4部分组成;眼柄内存在明暗相间的生长纹,样本生长纹的数目范围为8~31个;生长纹间距由色素层与钙化层的交界处到钙化层与膜层的交界处呈现出由宽到窄的变化趋势。克氏原螯虾眼柄的生长纹数目与其体长、体质量的Logistic关系式分别为L=(96.66)/(1+e~(-0.25(t-2.54)))、W=(22.48)/(1+e~(-0.41(t-11.78)))。

A study ongrowth of Procambarus clarkii based on eye stalk microstructure analysis

Crustaceans hard structures, which can record biological information, disappear periodically after molting.Thus, studyon the age and growth of crustaceans has been a tricky problem. In this paper,Procambarus clarkii were collected from the river channel of NanhuiNew City, PudongNew District, Shanghai Municipality. The eyestalks microstructure of the species wasanalyzed, and the relationship between growth incrementsinthe eyestalks and the length and weight of Procambarus clarkii were studied. Clear microstructures were observed both in cross and vertical section of the eyestalks, which are composed of four parts (ie. epicuticle, exocuticle, endocuticle and membranous layer).Each growth increments consist of one light and one dark increment.The number of growth increments of sampled specimens are form8 to 31.The width of growth increments become narrow fromthe junction of exocuticle and endocuticle to the junction of endocuticle and membranous layer.The number of growth increments in eyestalks and the length and weight of Procambarus clarkiiare both suit for Logistic relationships, and their functions are L=(96.66)/(1+e^{-0.25(t-2.54)}) and W=(22.48)/(1+e^{-0.41(t-11.78)}) respectively.