首页 | 本学科首页   官方微博 | 高级检索  
     检索      

拟南芥隐花色素突变体抑制子的筛选及其表型分析
引用本文:陈福禄,李宏宇,林辰涛,傅永福.拟南芥隐花色素突变体抑制子的筛选及其表型分析[J].中国农业科技导报,2009,11(3):93-97.
作者姓名:陈福禄  李宏宇  林辰涛  傅永福
作者单位:1.中国农业科学院作物科学研究所, 北京 100081, 中国,2.美国加州大学分子细胞与发育生物学系, 洛杉矶 90095, 美国
基金项目:国家自然科学基金项目 
摘    要:隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性。拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能。cry1cry2双突变体在长日条件下比野生型晚开花。采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D (suppressor of cry1cry2#17-Dominant)。TAIL-PCR结果表明,scc17-D突变体的T-DNA插入在第一条染色体上的At1g25440和At1g25450两个基因之间。scc17-D突变体表现出植株矮化、叶片变小、果荚变小和子叶表皮细胞形状发生改变等性状。

关 键 词:拟南芥  隐花色素  光周期  激活标签  开花  
收稿时间:2009-03-25
修稿时间:2009-05-07

Screening and Phenotypic Analysis of a Suppressor of Cryptochromes Mutant in Arabidopsis
CHEN Fu-lu,LI Hong-yu,LIN Chen-tao,FU Yong-fu.Screening and Phenotypic Analysis of a Suppressor of Cryptochromes Mutant in Arabidopsis[J].Journal of Agricultural Science and Technology,2009,11(3):93-97.
Authors:CHEN Fu-lu  LI Hong-yu  LIN Chen-tao  FU Yong-fu
Institution:1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2.Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
Abstract:Cryptochromes are flavoproteins that share similarity with the sequence of amino acids in DNA photolyase but lack photolyase activity. Among members of cryptochromes gene family in Arabidopsis thaliana, cryptochrome1 (CRY1) and CRY2 primarily mediate blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation, respectively, although their functions do overlap. The cry1cry2 double mutant flowered later than wild-type under long day conditions. Through activation tagging, we identified an early flowering mutant called scc17-D (suppressor of cry1cry2#17-Dominant). TAIL-PCR results showed that a T-DNA insertion located between At1g25440 and At1g25450 in the first chromosome is responsible for the scc17-D  mutant. The scc17-D  mutant displayed dwarfism, reduction in leaf size, reduced length of siliques, and the altered shape of cotyledon pavement cells.
Keywords:Arabidopsis thaliana  cryptochrome  photoperiod  activation tagging  flowering
本文献已被 维普 万方数据 等数据库收录!
点击此处可从《中国农业科技导报》浏览原始摘要信息
点击此处可从《中国农业科技导报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号