玉米胚性愈伤组织的遗传转化及耐草甘膦植株再生

以优良玉米自交系X2211的幼胚胚性愈伤组织为受体材料,采用农杆菌介导法将来自微生物荧光假单胞杆菌(Pseudomonas fluorescens)G2菌株的抗除草剂基因2mG2-epsps转入玉米愈伤组织细胞,转化后的细胞经草甘膦筛选,获得抗性愈伤组织并再生植株282株,成活86株,经特异PCR检测表明其中26株植株转入了2mG2-epsps基因,喷施0.125%除草剂试验检测表明,转基因植株具有良好的耐除草剂特性,为抗除草剂玉米新品种选育提供了较好的亲本材料。

Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant

2mG2-epsps gene from Pseudomonas fluorescens G2 was transferred to immature embryo-derived callus of the elite maize inbred line X2211 via Agrobacterium-mediated transformation. 282 transformed plants were regenerated from glyphosate-resistant callus, 86 plants of which were grown well in the field. Glyphosate resistance and allele-specific PCR analysis demonstrated that the 2mG2-epsps gene was transferred into the maize genome in 26 regeneration plants. The plants showed resistance after spraying 0.125% glyphosate, which indicated the expression of 2mG2-epsps gene in the plants. Some of the transgenic plants gained high tolerance to glyphosate. This study has provided good parental materials for breeding new glyphosate-resistant maize varieties.