| 棉花银染mRNA差异显示分析体系的建立及优化 |
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| 引用本文： | 安君,唐巧玲,王淼,王旭静,王志兴.棉花银染mRNA差异显示分析体系的建立及优化[J].中国农业科技导报,2008,10(Z1):90-93. |
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| 作者姓名： | 安君 唐巧玲 王淼 王旭静 王志兴 |
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| 作者单位： | 中国农业科学院生物技术研究所 中国农业科学院生物技术研究所 北京 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(中国农业科学院生物技术研究所)资助 |
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| 摘 要： | mRNA差异显示(mRNA differential display)技术是筛选差异表达基因的有效方法。本研究优化了棉花mRNA的银染差异显示体系,以陆地棉苏棉12为材料,分别提取叶片和开花期后10 d左右的纤维中的总RNA,利用差显技术筛选出了在棉花纤维中差异表达的cDNA片段。
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| 关 键 词： | 棉花 银染 mRNA差异显示 |
| 收稿时间： | 2008-01-28 |
| 修稿时间： | 2008-04-10 |
Establishment and Optimization of Sliver Staining Differential Display in Cotton |
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| AN Jun|TANG Qiao-Ling|WANG Miao|WANG Xu-Jing|WANG Zhi-Xing.Establishment and Optimization of Sliver Staining Differential Display in Cotton[J].Journal of Agricultural Science and Technology,2008,10(Z1):90-93. |
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| Authors: | AN Jun|TANG Qiao-Ling|WANG Miao|WANG Xu-Jing|WANG Zhi-Xing |
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| Institution: | Biotechnology Research Institute|Chinese Academy of Agricultural Sciences|Beijing 100081|China |
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| Abstract: | mRNA differential display technology is an effective method for screening differentially expressed genes.In this study,we optimized the silver staining differential display system in cotton.Total RNA were extracted from leaves and about 10 days DPA fiber of upland cotton Su 12.Differentially expressed cDNA fragments in fiber were selected by using differential display method. |
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| Keywords: | cotton sliver staining mRNA differential display |
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