| 一种新的水稻种子碱性脂肪酶活性快速检测方法及其应用 |
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| 引用本文： | 张瑛,吴跃进,何成芳,刘洁,蒋家月.一种新的水稻种子碱性脂肪酶活性快速检测方法及其应用[J].中国农业科技导报,2009,11(Z2):43-45. |
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| 作者姓名： | 张瑛 吴跃进 何成芳 刘洁 蒋家月 |
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| 作者单位： | (1.中国科学院等离子体物理研究所,安徽省离子束生物工程学重点实验室, 合肥 230031,2.安徽省农业科学院水稻研究所, 安徽省水稻遗传育种重点实验室, 合肥230031) |
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| 基金项目： | "十一五"国家科技支撑计划资助项目，安徽省优秀青年基金 |
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| 摘 要： | 微生物碱性脂肪酶研究及其应用均非常广泛,而关于水稻种子碱性脂肪酶却知之甚少。介绍一种新的水稻种子碱性脂肪酶活性平板检测方法,并利用该方法筛选碱性脂肪酶活性高低不同的材料以研究碱性脂肪酶在稻谷加速老化过程中的作用。结果表明碱性脂肪酶检测方法及其在稻谷人工老化过程中的作用对揭示稻谷储藏特性具有重要研究价值。
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| 关 键 词： | 水稻 种子 碱性脂肪酶 检测方法 加速老化 |
| 收稿时间： | 2009-08-04 |
| 修稿时间： | 2009-11-04 |
A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application |
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| ZHANG Ying,WU Yue-jin,HE Cheng-fang,LIU Jie,JIANG Jia-yue.A New Rapid Method for Estimating Rice Seed Alkaline Lipase and its Application[J].Journal of Agricultural Science and Technology,2009,11(Z2):43-45. |
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| Authors: | ZHANG Ying WU Yue-jin HE Cheng-fang LIU Jie JIANG Jia-yue |
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| Institution: | (1.Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031|2.Anhui Academy of Agricultural Sciences, Institute of Rice Research, Key Laboratory of Rice Genetics &|Breeding of Anhui Province, |Hefei |230031, China) |
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| Abstract: | It is extremely widespread about the research on microbial alkaline lipase and its application, but the rice seed alkaline lipase are poorly understood. The paper introduced a new plate examination method for estimating rice seed alkaline lipase activity. Using this method, rice seed materials with different alkaline lipase activity was screened, and used to study the function on alkaline lipase activity in an accelerated aging experiment. The result indicated that the method for eatimating alkaline lipase and its application in grain artificial aging are of great value to characters of grain storage. |
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| Keywords: | rice seed alkaline lipase plate examination method accelerated aging |
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