干旱胁迫对栀子内源激素含量的影响

为了探明栀子（Gardenia jasminoides Ellis）响应干旱胁迫过程中内源激素含量的变化，采用5%、10%和20%的聚乙二醇（PEG-6000）模拟轻度、中度与重度干旱胁迫，在胁迫开始的第5、10、15 d利用酶联免疫吸附法对栀子幼苗叶片4种内源激素的含量进行测定。结果表明:相较于对照处理，随着干旱胁迫时间的延长，轻度与中度干旱胁迫下栀子叶片脱落酸（abscisic acid，ABA）含量逐渐上升，重度胁迫下ABA含量先上升后下降；吲哚乙酸（indoleacetic acid，IAA）与赤霉素（gibberellic acid，GA）含量均先上升后下降；玉米素核苷（zeatin ribiside，ZR）含量呈现高-低-高的变化趋势；IAA/ABA、GA/ABA值逐渐下降，并且下降幅度较大；ZR/ABA、IAA/GA、IAA/ZR、GA/ZR值发生不同波动。综合分析，在遭受干旱胁迫后，栀子将会通过调节自身内源激素含量及比例来抵御干旱。

Effect of Drought Stress on Endogenous Hormone Content of Gardenia jasminoides Ellis

In order to study the change of endogenous hormones content of Gardenia jasminoides Ellis in response to drought stress, this paper measured the content of four kinds of endogenous hormones of Gardenia jasminoides Ellis leaves every 5 d by simulating light, moderate and severe drought stress with 5%, 10% and 20% polyethylene glycol (PEG-6000) . The results showed that: compared with normal treatment, with the extension of drought stress time, the content of abscisic acid (ABA) increased gradually in Gardenia jasminoides Ellis leaf under light and moderate drought stress, the contents of ABA increased first and then decreased under severe drought stress, the contents of indoleacetic acid (IAA) and gibberellic acid (GA) increased first and then decreased, the contents of zeatin riboside (ZR) had a trend of high-low-high. The values of IAA/ABA and GA/ABA decreased gradually, the values of ZR/ABA, IAA/GA, IAA/ZR and GA/ZR fluctuated differently. Comprehensive analysis showed that Gardenia jasminoides Ellis would response to drought by adjusting the content and proportion of endogenous hormones after drought stress.