| 来源于瓶霉Phialophora sp. G5的酸性木聚糖酶基因的克隆及性质的研究 |
| |
| 引用本文： | 张帆,石鹏君,缪礼鸿,姚斌.来源于瓶霉Phialophora sp. G5的酸性木聚糖酶基因的克隆及性质的研究[J].中国农业科技导报,2011,13(3):60-66. |
| |
| 作者姓名： | 张帆 石鹏君 缪礼鸿 姚斌 |
| |
| 作者单位： | (1.武汉工业学院生物与制药工程学院, 武汉 430023,2.中国农业科学院饲料研究所, 农业部饲料生物技术重点实验室, 北京 100081) |
| |
| 基金项目： | 国家转基因生物新品种培育重大专项，国家肉鸡产业技术体系 |
| |
| 摘 要： | 通过简并PCR和TAIL-PCR技术从瓶霉Phialophora sp. G5菌株基因组DNA中克隆得到一个新的编码b-1,4-木聚糖酶的基因,命名为xyn11G5。该基因ORF全长879 bp,共编码292个氨基酸和一个终止密码子,前19个氨基酸为信号肽序列。将xyn11G5在毕赤酵母中进行分泌表达,重组蛋白经纯化达到电泳纯。酶学性质分析表明,该重组木聚糖酶最适温度为50℃,最适pH为5.0,在中性条件下具有良好的稳定性,并且具有一定的热稳定性,在饲料行业和生物能源行业领域具有潜在的应用前景。
|
| 关 键 词： | Phialophora sp. G5 木聚糖酶 毕赤酵母 酶学性质 |
| 收稿时间： | 2011-02-22 |
Studies on Cloning of Acid Xylanase from Phialophora sp.G5 and its Characteristics |
| |
| ZHANG Fan,SHI Peng-jun,MIAO Li-hong,YAO Bin.Studies on Cloning of Acid Xylanase from Phialophora sp.G5 and its Characteristics[J].Journal of Agricultural Science and Technology,2011,13(3):60-66. |
| |
| Authors: | ZHANG Fan SHI Peng-jun MIAO Li-hong YAO Bin |
| |
| Institution: | (1.School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023|2.Key Laboratory for Feed Biotechnology, Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China ) |
| |
| Abstract: | A xylanase gene xyn11G5 was cloned from Phialophora sp. G5 by degenerate PCR and TAIL-PCR method. Its ORF was 879 bp, encoded 292 amino acids residues, and the frontal 19 residues composed a putative signal peptide. Xyn11G5 was successfully expressed in Pichia pastoris and purified. The enzymatic characteristics analysis showed that the optimum pH and temperature for the recombinant xylanase was pH 5.0 and 50℃, respectively. It showed pH stability under neutral and thermo stability. Xyn11G5 was a good candidate in animal feed and bio-fuel industrial application. |
| |
| Keywords: | Phialophora sp G5 b-1 4-xylanase Pichia pastoris enzyme characteristics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科技导报》浏览原始摘要信息 |
| 点击此处可从《中国农业科技导报》下载免费的PDF全文 |
|
