磷脂分析方法与应用研究进展

磷脂是所有活体细胞膜的主要成分,具有重要的结构和功能特性。对其进行高效分析,有助于深入理解特定的磷脂如何在正常生理或疾病状态发挥作用,更能为磷脂的生物学特性和营养特性的深入研究提供技术平台。由于自然界和生物体内存在的磷脂种类繁多,且每种磷脂骨架上可以结合的脂肪酸很多,导致其组成变化大,对磷脂的分析一直比较困难。随着分析技术的发展,磷脂分析方法的研究越来越多,综述了磷脂的结构及生物学功能、提取方法、分离方法、质谱鉴定方法、定量分析方法及在脂质组学中的应用等几个方面,并对磷脂分析的发展方向进行了展望,以期为磷脂分析方法的建立,以及磷脂生物学特性和营养特性的深入研究提供参考。

Phospholipid Profiling Method and Progress on its Application Research

Phospholipid is a main component of all living cell membranes, and has important structural and functional features. Phospholipid high efficient profiling is helpful for deeper understanding the role of particular phospholipid played in maintaining healthy or sick state of a cell; and it also provides a technology platform for in-depth study on the biological and nutritional characteristics of phospholipid. Analyzing phospholipid has become more difficult, because of its complex structure and species in nature and in vivo. With the development of analyzing technology, many studies are carried out on the analyzing methods. This paper summarized the structure and biological function of phospholipid, methods for its extraction, separation, mass spectrum identification, quantitative analysis, and application in lipidomics. The development direction of phospholipid analisis was prospected, so as to provide references for establishing new phospholipid analyzing method, and for further studying its biological and nutritional characteristics.