| 农杆菌介导多年生黑麦草转化体系的建立 |
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| 引用本文： | 王奇丽,何近刚,陈彦龙,张小芸,吴金霞.农杆菌介导多年生黑麦草转化体系的建立[J].中国农业科技导报,2009,11(2):119-123. |
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| 作者姓名： | 王奇丽 何近刚 陈彦龙 张小芸 吴金霞 |
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| 作者单位： | (1.山西农业大学, 山西 太谷 030801,2.中国农业科学院生物技术研究所, 北京 100081) |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 以胚性愈伤组织系,作为转化受体,利用农杆菌介导的遗传转化方法,将报告基因gus和抗除草剂基因bar
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| 关 键 词： | 多年生黑麦草 农杆菌介导转化 GUS染色 PCR检测 Basta筛选 |
| 收稿时间： | 2008-11-24 |
| 修稿时间： | 2008-12-05 |
Establishment of an Agrobacterium Mediated Transformation
System for Lolium perenne L. |
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| WANG Qi-li,HE Jin-gang,CHEN Yan-long,ZHANG Xiao-yun,WU Jin-xia.Establishment of an Agrobacterium Mediated Transformation
System for Lolium perenne L.[J].Journal of Agricultural Science and Technology,2009,11(2):119-123. |
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| Authors: | WANG Qi-li HE Jin-gang CHEN Yan-long ZHANG Xiao-yun WU Jin-xia |
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| Institution: | (1. Shanxi Agricultural University, Shanxi Taigu 030801|;
2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081, China) |
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| Abstract: | The vector pDM805 which harbors the selection gene bar and reporter gene gus was
transformed into embryogenic callus lines of ryegrass. In total, 52 individual
regenerated plants were obtained from bialaphos resistant callus lines. Among them, 27
were confirmed by bar PCR analysis; 15 of the 27 PCR positive plants showed strong blue
color with GUS staining. The results suggested that through optimizing callus induction
and plant regeneration conditions, an efficient Agrobacterium mediated transformation
protocol was established in ryegrass, which paves the way for its genetic improvement
through genetic engineering in the near future. |
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| Keywords: | perennial ryegrass Agrobacterium mediated transformation GUS staining PCR detection Basta selection |
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