| 高效液相色谱法测定灵芝多糖含量 |
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| 引用本文： | 韩振泰,赵玉娟,刘惠文,李如华.高效液相色谱法测定灵芝多糖含量[J].中国农业科技导报,2009,11(Z1):65-67. |
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| 作者姓名： | 韩振泰 赵玉娟 刘惠文 李如华 |
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| 作者单位： | (中国林科院森林生态环境与保护研究所, 国家林业局森林生态环境重点实验室, 北京 100091) |
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| 基金项目： | 本方法由中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 建立了高效液相色谱法测定灵芝多糖含量的方法,采用糖专用分析柱,以104分子量的葡聚糖为标准,流动相为水,流速0.6 mL/min,检测器为示差检测器,柱温为70℃,检测限为 0.03 g/L,线性方程为Y=109 779.6X-903.3相关系数r=0.999 9,方法回收率为98%～104%,相对标准偏差为RSD%=0.12(n=6)。该方法简易、稳定,适合灵芝多糖的测定。
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| 关 键 词： | 高效液相色谱 示差检测器 灵芝 多糖 |
| 收稿时间： | 2009-08-15 |
| 修稿时间： | 2009-10-28 |
Determination of Ganoderma Lucidum Polysaccharide by Reversed-phase High Performance Liquid Chromatography |
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| HAN Zhen-tai,ZHAO Yu-juan,LIU Hui-wen,LI Ru-hua.Determination of Ganoderma Lucidum Polysaccharide by Reversed-phase High Performance Liquid Chromatography[J].Journal of Agricultural Science and Technology,2009,11(Z1):65-67. |
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| Authors: | HAN Zhen-tai ZHAO Yu-juan LIU Hui-wen LI Ru-hua |
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| Institution: | (Key Laboratory of Forest Ecological Environment of Ministry of Forestry|Research Institute of Forestry Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China) |
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| Abstract: | A reversed-phase high performance liquid chromatographic method (HPLC) for the determination of ganoderma lucidum polysaccharide was developed, using SUGUR PARK-Ⅰ(6.5×300 mm) as the analysis column, glucan 10 000 as standard, water as mobile phase at a flow rate of 0.6 mL/min. The differential refractometer detector was used and the column temperature was 70℃. The limit of detection was 0.03 g/L. The linear regression equation was Y=109 779.6X－903.3, the correlation coefficient r=0.999 9. The method recoveries ring from 98% to 104%. Relative standard deviation was 0.12% (n=6). The method is simply, stable, suitable for the determination of ganoderma lucidum polysaccharide. |
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| Keywords: | high performance liquid chromatography differential refractometer detector ganoderma lucidum polysaccharide |
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