玉米低聚肽对CCl_4所致小鼠急性肝损伤的保护作用研究

为了研究玉米低聚肽对CCl4所致小鼠急性肝损伤的保护作用,对小鼠经口灌胃200 mg/kg·d、400 mg/kg·d、800 mg/kg·d的玉米低聚肽进行肝损伤前预防,30 d后,以10 m L/kg的剂量一次性灌胃0.5%的CCl4,16 h后测定小鼠的肝指数、肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px);血清中谷丙转氨酶(ALT)、谷草转氨酶(AST),光镜下观察肝组织学变化。结果显示玉米低聚肽中、高剂量组均能显著降低CCl4所致急性肝损伤小鼠的ALT、AST和MDA水平(P0.05),提高SOD和GSHpx活力(P0.05),且明显促进肝功能恢复,光镜下可见玉米低聚肽明显改善肝组织损伤程度,但对肝指数无明显影响。上述结果表明玉米低聚肽对CCl4所致小鼠急性肝损伤有保护作用。

Studies on Protective Effect of Corn Oligopeptide Against Acute Hepatic Injury of Mouse

To explore the protective effect of corn oligopeptide(COP) on CCl4-induced acute hepatic injury of mouse, this experiment carried out precautions for liver injury by intragastric infusion orally with COP daily dose of 200 mg/kg·d, 400 mg/kg·d, 800 mg/kg·d to mice. After 30 d, a dose of 10 mg/kg and 0.5% CCl4 was intragastric infused. 16 h later mouse liver index, the value of malondialdehyde (MDA), superoxide dismutase (SOD), glutathion peroxidase (GSH-px) and the contents of serum alanine aminotransferase (ALT) and glutamic-oxalacetic transaminease(AST) were measured. The pathological changes in hepatic tissues of each group were observed. The result showed that COP at high and middle doses could all significantly reduce the levels of AST, ALT and MDA of liver injured mouse caused by CCl4 (P<0.05) and improved their SOD and GSH-Px vigor (P<0.05). COP could also promote the recovery of mouse liver function, but had little effect on liver index. Under microscope, it could be observed that COP improved mouse hepatic injury. The above results indicated that COP can protect CCl4-induced acute liver damage of mouse.