大豆耐低磷指标筛选与耐低磷品种鉴定

为了对大豆耐低磷指标的进行分析与评价,并筛选与大豆磷效率耐低磷性密切相关的重要指标,选取了41个大豆品种进行了耐低磷系数进行比较和分析,并对酸性磷酸酶活性与相应的株高、干物质量和磷利用效率的耐低磷系数利用NTSYS软件进行聚类分析。结果表明:12μmol/L(LP)和1 000μmol/L(NP)处理下22个品种根尖酸性磷酸酶(RAPA)的耐低磷系数(1.922 9~35.201 1),32个品种LAPA的耐低磷系数(1.048 2~4.465 5),9个品种株高的耐低磷系数(0.923 1~1.000 0),17个品种干物质量的耐低磷系数(1.000 0~2.125 0),28个品种全磷含量(P%)的耐低磷系数(1.083 2~6.757 3),18个品种磷利用效率的耐低磷系数(1.005 4~3.308 6),不同基因型品种中耐低磷系数差异大;2RAPA活性与相应品种的株高、干物质量和磷利用率之间耐低磷系数存在极显著正相关(r=0.55、0.72、0.43);3RAPA活性和株高、干物质量、磷利用效率等磷效率相关因素的耐低磷系数进行聚类分析,鉴定出高度、中度和非耐低磷型品种。上述结果说明RAPA活性耐低磷系数可作为影响磷效率耐低磷性的重要因素,可用于大豆耐低磷品种的筛选及分子辅助选择。

Screen Indexes for Soybean Tolerance to Phosphorus Deficiency and Identification of Low Phosphorus Tolerant Soybean Cultivars

In order to analyze and evaluate the indexes of soybean tolerane to low phodphalt, and screen the important targets closely related to soybean tolerance to low phosphorus, this paper selected 41 soybean cultivars, and compared and analyzed their coefficient of low phosphorus tolerance, and carried out clustering analysis by NTSYS software on acid phosphatase activity of root tip with relavant plant high, amount of dry matter with low-P tolerance coefficient of phosphrous utilization rate. The results under the treatments of 2 umol/L (LP) and 1 000 umol/L (NP) showed: ①there were big differences in low-P tolerance coefficients among varieties with different genotypes: the low phosphorus tolerance coefficient of acid phosphatase activity of root tip (RAPA) of 22 varieties were 1.922 9~35.201 1, and low phosphorus tolerance coefficient of acid phosphatase activity of leaf blade (LAPA) of 32 species were 1.048 2~4.465 5，and plant high low phosphorus tolerance coefficient of 9 varieties were 0.923 1~1.000 0, and the phosphorus tolerance coefficient of dry matter amount of 17 varieties were 1.000 0~2.125 0, and low phosphorus tolerance coefficient of total phosphorus content (P%) of 28 species were 1.083 2~6.757 3, and low phosphorus tolerance coefficient of phosphorus utilization rate of 18 species were 1.005 4~3.308 6; ②there was significantly positive correlation (r=0.55,0.72,0.43) among RAPA, plant height, dry matter amount of related varieties and Phosphorous utilization efficiency with the coefficient of low phosphorus tolerance. ③The clustering analysis on the coefficient of low phosphorus tolerance between RAPA, plant height, dry matter amount, phosphorous utilization efficiency, etc. Phosphorous efficiencyrelated factors had identified soybean varieties with high, medium and no tolerance to low phosphorus. The above results indicated that the coefficient of low phosphorus tolerance of RAPA could be used as an important factor to affect low phosphorus tolerance of phosphorous efficiency, and also as a molecular assistant to screen soybean varietis with tolerance to low phosphorus.