芥蓝芥子油苷提取条件优化研究

采用正交试验设计,研究了提取温度、提取剂甲醇浓度(V/V)、提取时间和待测物质与提取液比例(简称"物液比")对芥蓝菜薹中芥子油苷(总芥子油苷含量、总脂肪族芥子油苷和总吲哚族芥子油苷)提取效果的影响。结果表明,物液比和提取温度对总芥子油苷、总脂肪族芥子油苷和总吲哚族芥子油苷提取效果影响显著(P0.05),而甲醇浓度和提取时间对芥子油苷提取率无显著影响(P0.05)。综合考虑提取效果和成本,芥蓝中芥子油苷最佳提取条件为75℃,物液比1∶45(g∶mL),甲醇浓度70%,提取时间5 min。在该条件下,总芥子油苷提取量为8.31μmol/g DW,总脂肪族芥子油苷提取总量为7.58μmol/g DW,总吲哚族脂肪族芥子油苷提取总量为0.73μmol/g DW。

Studies on Optimization of Extracting Condition for Glucosinolates in Chinese Kale (Brassica alboglabra Bailey)

Taking the orthogonal test design, this paper studied the effect of different extraction factors (extract temperature, methanol concentrations of extract solution, extract time, and ratios between sample to be tested and extract solution) on glucosinolates (GSs) extraction the total content of GSs, total aliphatic GSs, and total indolyl GSs from bolting stems of Chinese kale (Brassica alboglabra Bailey). Sample/solution ratio and extract temperature had significant influence on the total content of GSs, total aliphatic GSs, and total indolyl GSs (P<0.05). While, methanol concentration and extract time had no significant influence on Glucosinolate extraction yield (P>0.05). Considering the extraction effect and cost, we suggested the optimal extract temperature, ratio of sample to extract solution, methanol concentrations of extract solution, and extract time are 75℃, 1∶45(g∶mL), 70% and 5 min, respectively. Under the optimal condition, the total content of GSs, total aliphatic GSs, and total indolyl GSs are 8.31 μmol/g DW, 7.58 μmol/g DW, and 0.73 μmol/g DW, respectively.