沙蚕不同酶解产物抗氧化效果研究

海洋生物蛋白资源是海洋生物资源的重要组成部分，对其进行高值化加工利用是海洋生物技术研究的重要内容，酶解是提高海洋蛋白功能性的有效方式，但酶种类不同,其作用位点不同，可能对酶解产物的活性有一定影响。为了探究了沙蚕不同酶解产物的抗氧化性，采用6种蛋白酶（碱性蛋白酶、中性蛋白酶、胃蛋白酶、木瓜蛋白酶、风味蛋白酶、胰蛋白酶）酶解沙蚕，测定了不同酶解时间下的水解度、分子量分布、3种自由基（DPPH、O-2·、·OH）的清除率等反映抗氧化水平的指标。结果表明：胰蛋白酶小分子肽得率最高，为70.47%；胃蛋白酶次之，为68.12%。酶解液浓度为5 mg·mL-1时，胃蛋白酶和木瓜蛋白酶酶解4 h时，DPPH清除率效果较好，分别为90.87%和90.52%。胃蛋白酶酶解3 h时，O-2·清除率效果最佳，为89.78%；木瓜蛋白酶酶解3 h时，O-2·清除率效果次之，为57.99%。碱性蛋白酶酶解4 h和胃蛋白酶酶解2 h时，·OH清除率效果较好，分别为85.07%和83.37%。胰蛋白酶和胃蛋白酶对提高酶解液水解度和小肽生成作用明显，胃蛋白酶的酶解产物对3种自由基清除率较好，更适用于制备沙蚕抗氧化肽。

Research on the Antioxidant Effect of Different Enzymatic Hydrolysis Products of Nereis succinea

Marine biological protein resources are important parts of marine living resources. High-value processing and utilization of marine biological resources is important for marine biological technology research. Enzymatic hydrolysis is an effective way to improve the function of marine proteins. However, different types of enzymes have different action sites, which may have certain effects on the activity of enzymatic hydrolysis products. In order to explore the antioxidant properties of different enzymatic hydrolysis products of Nereis succinea, 6 protease (alkaline protease, neutral protease, pepsin, papain, flavor protease, trypsin) were used to enzyme Nereis succinea, and some indexes reflecting the level of antioxidant, such as the degree of hydrolysis, molecular weight distribution and the scavenging rate of 3 kinds of free radicals (DPPH, O-2·, ·OH) were measured. The results showed that trypsin had the highest yield of small molecule peptides at 70.47%, and the pepsin was second with 68.12%. At the concentration of 5 mg·mL-1, pepsin and papain had better effects on DPPH scavenging rate of 90.87% and 90.52% after 4 h enzymatic hydrolysis, respectively. The best effect on O-2· scavenging rate was 89.78% after 3 h pepsin hydrolysis. Papain had the second best effect on O-2· scavenging rate after 3 h enzymatic hydrolysis, which was 57.99%. The effects of alkaline protease hydrolysis for 4 h and pepsin hydrolysis for 2 h on the ·OH scavenging rate were good, which were 85.07% and 83.37%, respectively. Trypsin and pepsin had obvious effects on improving the hydrolysis degree of enzymatic hydrolysate and the production of small peptide, and pepsin hydrolysis had better scavenging rate for 3 kinds of free radicals, which indicated that pepsin was more suitable for the preparation of antioxidation peptide from Nereis succinea.