响应面试验优化霍山石斛原球茎增殖培养基

为可持续开发霍山石斛资源，采用响应面法优化霍山石斛原球茎增殖培养基。在单因素试验基础上，根据Box Behnken试验设计原理，采用三因素三水平的响应面分析法，以增殖率为响应值进行回归分析。结果表明，萘乙酸（简称NAA）及6 苄氨基嘌呤（简称6 BA）浓度对增殖率影响最为显著，6 BA与马铃薯两者间交互作用显著，适宜的NAA、6 BA及马铃薯浓度分别为0.05 mg·L-1、0.70 mg·L-1和185 g·L-1，此时增殖率为2 989.82%±205.55%，与模型预测值基本符合。响应面法优化霍山石斛原球茎增殖培养基切实可行。

Optimization of Protocorm Proliferation Medium of Dendrobiumhuoshanense by Response Surface Methodology

In order to explore sustainable development of Dendrobium huoshanense resources, this paper used response surface method to optimize the protocorm multiplication medium. On the basis of single factor tests, the mathematical regression model was established about the dependent variable (proliferation rate) through Box Benhnken center composite design and response surface analysis. The results showed that the concentration of NAA and 6 benzylaminopurine (6 BA) had the most significant effect on the proliferation rate, and the interaction between 6 BA and potato was significant. The suitable concentrations of NAA, 6 BA and potato were 0.05 mg·L-1, 0.70 mg·L-1 and 185 g·L-1, respectively. At this time, the proliferation rate was 2 989.82% +205.55%, which was consistent with the predicted value of the model. Above results indicated that the response surface methodology was viable for optimizing medium composition in improving proliferation rate.