新疆库尔勒地区羊源产志贺毒素大肠杆菌及其耐药性分析

为了解新疆库尔勒地区羊源产志贺毒素大肠杆菌（Shiga toxin-producing Escherichia coli，STEC）毒力基因的分布情况及其耐药性，对新疆库尔勒地区羊肉生产环节（养殖场、定点屠宰场和市场）的282份样品进行STEC的分离鉴定，并检测毒力基因的编码情况，进一步检测了17种常用抗生素的耐药情况。结果表明：羊肉生产环节样品中检出72株STEC，检出率为25.5%。72株STEC中编码毒力基因stx1+stx2、stx1、stx2、rfbE、eae、hly的数量分别为22、45、2、0、4、51个。其中，羊养殖场中存在较多的非O157 STEC，检出率为37.3%；stx以stx1为主，检出率达到了652%。72株STEC中以β-内酰胺类、氨基糖苷类、大环内酯类抗生素耐药为主，STEC菌株的多重耐药比较严重，最多可耐9种抗生素，多重耐药情况主要是耐1种和耐2种抗生素。研究表明，羊肉生产环节中羊养殖场存在大量的非O157 STEC菌株，且耐药情况比较严重，务必预防源头污染。

Analysis of Drug Resistance and the Shiga Toxin-producing Escherichia coli in Sheep from Korla Region of Xinjiang

In order to understand the distribution of the Shiga toxin-producing Escherichia coli (STEC) and its virulence genes from sheep in Korla region of Xinjiang, the isolation and identification of STEC on 282 samples from sheep farms, slaughterhouses and markets in Korla region of Xinjiang were carried out, and the coding of virulence genesand the drug resistance of STEC strain to 17 common antibiotics was detected. The results showed that: 72 strains of STEC were detected in mutton production of 282 samples with 25.5% of detection rate. The number of virulence genes stx1+stx2, stx1, stx2, rfbE, eae and hly in 72 STEC strains were 22, 45, 2, 0, 4, 51, respectively. Among them, there were more non-O157 STEC in the sheep farm and the detection rate was 37.3%. stx1 gene was Shiga toxin mainly and the detection rate reached 65.2%. Drug resistance was dominated from β-lactam antibiotics, aminoglycoside antibiotics and macrolide antibiotics in the 72 STEC strains. The STEC strain had severe multiple drug resistance and could be resistant to nine antibiotics. Multiple drug resistance was mainly resistance to one and two antibiotics. This research showed that there were a large number of non-O157 STEC strains in sheep farms in the mutton production process, and the drug resistance was more serious, it was necessary to prevent source pollution.