| 四种转基因玉米新型质粒标准分子协同实验验证 |
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| 引用本文： | 沈愷琳,李想,王姝,张大兵,杨立桃.四种转基因玉米新型质粒标准分子协同实验验证[J].中国农业科技导报,2009,11(5):90-95. |
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| 作者姓名： | 沈愷琳 李想 王姝 张大兵 杨立桃 |
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| 作者单位： | （1.上海交通大学生命科学技术学院陆伯勋食品安全中心转基因作物检测实验室, 上海 200240;2.上海海洋大学水产与生命学院, 上海 201303;3.上海出入境检验检疫局动植物与食品检验检疫技术中心, 上海 200135） |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 构建了分别适于转基因玉米GA21、TC1507、T25和Bt11品系特异性双重PCR检测标准分子,并对其在定性PCR检测体系中的适用性进行了实验室间协同实验验证。对7家实验室返回结果的统计分析表明,标准分子pBt11、pTC、pT25和pGA分别对转基因玉米Bt11、TC1507、T25和GA21品系具有高特异性。4个标准分子在zSSⅡb和对应品系特异性序列单一PCR检测体系中检测下限均为50拷贝。因此,4个标准分子均可作为新型标准物质用于相应转基因玉米品系特异性定性检测。
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| 关 键 词： | 标准分子 品系特异性 协同实验 转基因玉米 |
| 收稿时间： | 2009-04-29 |
| 修稿时间： | 2009-06-12 |
Inter-laboratory Validation of Four Novel Plasmid Reference |Molecules Suitable For Four Genetically Modified Maize Event-specific Qualitative Detections |
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| SHEN Kai-lin,LI Xiang,WANG Shu,ZHANG Da-bing,YANG Li-tao.Inter-laboratory Validation of Four Novel Plasmid Reference |Molecules Suitable For Four Genetically Modified Maize Event-specific Qualitative Detections[J].Journal of Agricultural Science and Technology,2009,11(5):90-95. |
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| Authors: | SHEN Kai-lin LI Xiang WANG Shu ZHANG Da-bing YANG Li-tao |
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| Institution: | (1.GMO Detection Laboratory, SJTU-Bor Luh Food Safety Center, School of life Science and Biotechnology, Shanghai ;
Jiao Tong University, Shanghai 200240|2.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai ;
201303|3.GMO Detection Laboratory, Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China) |
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| Abstract: | In this study, reference molecules suitable for event-specific qualitative detection of genetically modified (GM) maize Bt11, TC1507, T25 and GA21, respectively, were constructed and validated by inter-laboratory validation. The statistical analysis of reported data from seven labs showed that pBt11, pTC, pT25 and pGA were highly specific to GM maize Bt11, TC1507, T25 and GA21, respectively. The limits of detection that could be detected by all participants were all 50 copies DNA of each reference molecule for the detection of zSSⅡb and event-specific sequence in qualitative PCR detection systems. All results indicated that the four constructed reference molecules can be used as reference materials for qualitative event-specific detection of the corresponding GM maize events. |
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| Keywords: | reference molecule event-specific inter-laboratory trail genetically modified maize |
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