首页 | 本学科首页   官方微博 | 高级检索  
     检索      

LipofectamineTM和Fugene-6介导GFP基因转染绵羊胎儿成纤维细胞的比较研究
引用本文:马玉珍,王瑞,闫真,王利民,刘东军,夏国良.LipofectamineTM和Fugene-6介导GFP基因转染绵羊胎儿成纤维细胞的比较研究[J].中国农业科技导报,2008,10(1):108-112.
作者姓名:马玉珍  王瑞  闫真  王利民  刘东军  夏国良
作者单位:[1]中国农业大学生物学院,北京100094 [2]内蒙古大学哺乳动物繁殖生物学与生物技术教育部重点实验室,呼和浩特010021
基金项目:国家自然科学基金 , 新疆生产建设兵团基金
摘    要:分别用Lipofectamine^TM和Fegene-6介导质粒pEGFP-C1转染体外培养的绵羊胎儿成纤维细胞(sheepfetal fibroblast cells,sFFCs),比较了DNA浓度、转染试剂用量以及细胞暴露于DNA、转染试剂中作用时间对转染效率的影响,通过含G418的DMEM/F12培养液筛选得到转基因单克隆细胞。结果表明脂质体转染试剂Lipofectamine^TM转染效率优于Fegene-6。另外,对转基因细胞进行了染色体核型分析。结果表明,转基因细胞中二倍体核型占74.5%,与对照组比较没有显著性差异。以上研究为其他基因转染sFFCs以及利用体细胞克隆法生产转基因绵羊提供了参考依据。

关 键 词:绵羊胎儿成纤维细胞  脂质体  转基因  染色体  
文章编号:1008-0864(2008)01-0108-05
收稿时间:2007-12-29
修稿时间:2007年12月29

Comparison Between Sheep Fetal Fibroblast Cells transfected with |GFP by Lipofectamine^TM and by Fugene-6
MA Yu-zhen,WANG Rui,YAN Zhen,WANG Li-min,LIU Dong-jun,XIA Guo-liang.Comparison Between Sheep Fetal Fibroblast Cells transfected with |GFP by Lipofectamine^TM and by Fugene-6[J].Journal of Agricultural Science and Technology,2008,10(1):108-112.
Authors:MA Yu-zhen  WANG Rui  YAN Zhen  WANG Li-min  LIU Dong-jun  XIA Guo-liang
Institution:1. College of Biological Science, China Agricultural University, Beijing 100094|2. Key Laboratory of Mammal Reproduetive |Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Huhhot 010021, China
Abstract:Sheep fetal fibrobast cell were transfected with pEGFP-C1 by using Lipofectamine^TM or Fugene-6 substance respectively. The impacts of DNA concentration, concentration of transfeetion agents, exposure time and incubation time on transfection efficiency were carefully compared. The transfected cells were incubated in DMEM/F12 media containing G418. The results showed that the efficiency of transgenic with LipofectamineTM were excelled Fugene-6. Chromosome analysis shows no remarkable difference between transgenic and normal cells. LipofectamineTM transfection method developed in this paper can be easily adopted by most research labs and will provide a basic reference for transfection of sheep fetal flbroblast ceils with other gene and transgenie nuclear transfer.
Keywords:sheep fetal fibroblast cell  liposome  gene transfer  chromosome
本文献已被 维普 万方数据 等数据库收录!
点击此处可从《中国农业科技导报》浏览原始摘要信息
点击此处可从《中国农业科技导报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号