| 盐爪爪根际耐盐促生菌的筛选及穴栽验证 |
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| 引用本文： | 张志东,顾美英,唐琦勇,楚敏,朱静,孙建,杨蓉,徐万里.盐爪爪根际耐盐促生菌的筛选及穴栽验证[J].中国农业科技导报,2021,23(3):186-192. |
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| 作者姓名： | 张志东 顾美英 唐琦勇 楚敏 朱静 孙建 杨蓉 徐万里 |
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| 作者单位： | 1.新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐 830091;
2.新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐 830091 |
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| 基金项目： | 国家自然科学基金项目（31560034）；
农业农村部西北绿洲农业环境重点实验室开放课题项目(XBLZ-20201)；
新疆农业科学院农业科技创新平台能力提升建设专项（NKYPT005）；
新疆农业科学院重点项目前期预研专项（XJZDY-004）；
新疆自治区“天山雪松计划”项目（2017XS26） |
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| 摘 要： | 为了筛选盐爪爪（Kalidium foliatum）根际耐盐促生菌，探索其对棉花耐盐促生的作用，采集了新疆和硕地区的盐爪爪根际土样，进行了植物根际促生菌的筛选，并开展了菌株的分子鉴定及棉花耐盐促生效果的分析。结果显示：试验共获得57株细菌，经分子鉴定确定其归属于细菌域4个门12个属。其中，放线菌门(Actinobacteria)所含菌属种类最多，共涉及6个属；其次为变形杆菌门(Proteobacteria)，涉及3个属。通过定性和定量分析发现，所得多数菌株具有耐盐促生作用。棉花穴栽验证试验结果表明，采用R11、T25、K18等菌株处理后，棉花发芽率均呈现不同程度的提高，对植株和根的促生长效果明显，其中R11菌株处理效果最好，其棉花发芽、株高和根长与对照相比分别提高了25.0%、135.2%和46.1%。综上所述，盐爪爪根际中存在着丰富多样的植物耐盐促生菌，研究结果为进一步开发耐盐促生微生物菌剂奠定了基础。
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| 关 键 词： | 盐爪爪 根际微生物 耐盐促生菌 穴栽试验 |
| 收稿时间： | 2019-09-03 |
Screening of Salt-tolerant and Growth-promoting Bacteria in the Rhizosphere of Kalidium foliatum and the Functional Identification in Pot Experiments |
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| ZHANG Zhidong,GU Meiying,TANG Qiyong,CHU Min,ZHU Jing,SUN Jian,YANG Rong,XU Wanli.Screening of Salt-tolerant and Growth-promoting Bacteria in the Rhizosphere of Kalidium foliatum and the Functional Identification in Pot Experiments[J].Journal of Agricultural Science and Technology,2021,23(3):186-192. |
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| Authors: | ZHANG Zhidong GU Meiying TANG Qiyong CHU Min ZHU Jing SUN Jian YANG Rong XU Wanli |
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| Institution: | 1.Xinjiang Special Environmental Microbiology Laboratory, Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 2.Institute of Soil Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China |
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| Abstract: | In order to screen the salt-tolerant and growth-promoting bacteria in the rhizosphere of Kalidium foliatum, and explore its effect on the salt-tolerant and growth-promoting of cotton, this paper collected rhizosphere soil samples from Heshuo County in Xinjiang to screen the salt-tolerant and growth-promoting bacteria, and carried out molecular identification of the strains and analysis of the salt-tolerant and salt-promoting effect of cotton. The results showed that: a total of 57 isolates were obtained and identified which were classified into 12 genera of 4 phyla. Among them, Actinobacteria contained the largest number of bacterial genera, involving 6 genera in total. The second was Proteobacteria, involving three genera. Functional analysis showed that most of strains exhibited characteristics of salt-tolerant and plant growth-promoting. In pot experiments, treatments with bacteria such as R11, T25 and K18, could increase germination percentage of cotton seeds to a certain degree, and obviously enhance the growth rate and root length of plants. In particularly, effects of treatment with strain R11 were marked in which germination rate, plant height and root length of cotton increased by 25%, 135.2% and 46.1%, respectively. In conclusion, there were various salt-tolerant and growth-promoting bacteria in the rhizosphere of Kalidium foliatum, which provided a solid foundation for the further development of salt-tolerant and growth-promoting microbial agents. |
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| Keywords: | Kalidium foliatum rhizosphere bacteria salt-tolerant and growth-promoting bacteria pot experiments |
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