山东地区樱桃褐斑病病原鉴定及rDNA-ITS序列分析

为明确引起樱桃褐斑病的病原菌种类,采集泰安、潍坊、烟台3个地区典型发病叶片,用单孢分离法分离、纯化,共获得16个菌株,采用病原菌形态学与分子生物学相结合的方法,鉴定樱桃褐斑病的病原菌。对该病原菌的ITS序列分析显示,其与Gen Bank中登录的Passalora sp.属的Passalora arctostaphyli相似性达97%;与另一种在甜樱桃叶片中分离的病原菌Pseudocercospora pruni-persicicola相似度仅为85.6%,证明两者并非为同一属真菌。构建系统进化树表明,樱桃褐斑病病原与Passalora sp.属真菌处在同一分支,而与其它Prunus sp.属分离的病原菌处在两个分支,进而通过形态学鉴定和ITS序列分析认为,病原菌为Passalora circumscissa,并将其ITS基因序列提交至Gen Bank数据库(基因登陆号:KT428056)。

Identification and ITS Sequence Analysis of Pathogen of Cherry Brown Leaf Spot in Shandong

In order to clarify the pathogenic species of cherry brown leaf spot, this study isolated and purified 16 fungal strains by single spore separation method. It also collected typical morbidity leaf blades from Taian, Weifang and Yantai 3 regions. Identification of cherry brown leaf spot pathogen was carried out by pathogen morphology combined with molecular biology method. The ITS sequence analysis of this pathogen showed that it was aligned with Genbank and showed 97% identity with Passalora arctostaphyli. The results of phylogenetic analysis showed that the pathogen was located at the same branch of phylogenetic tree with Passalora sp. and different from Pseudocercospora sp. According to its morphological characteristics and the sequence of internal transcribed spacer, the pathogen was identified as Passalora circumscissa, and ITS gene sequence was presented to GenBank database(Genbank Accession No.: KT428056).