菜籽粕芥子碱降压及其对血管内皮因子的影响

为考察油菜籽粕芥子碱氯化盐的降压作用及其对血管内皮因子的影响,采用动脉插管方式记录新西兰大耳白兔的收缩压、舒张压、平均动脉压研究芥子碱氯化盐的降压作用,酶联免疫吸附测定法(ELISA)检测血清中前列环素(PGI2)、血栓素A2(TXA2)、内皮素-1(ET-1)的水平,硝酸还原酶法检测血清中NO的水平。结果表明:芥子碱氯化盐在0.025~12.5 mg/kg浓度范围能够显著降低实验动物收缩压、舒张压及平均压;随其浓度增加,降压率上升,维持时间延长。ELISA实验结果表明芥子碱氯化盐对实验动物血清中PGI2、ET-1水平作用不显著,2.4 mg/kg与9.6 mg/kg芥子碱氯化盐能够显著降低血清中TXA2的水平。上述结果说明油菜籽粕芥子碱氯化盐具有显著的降压作用,其降压机制可能是通过抑制血管收缩因子TXA2的水平实现的。

Antihypertensive Effect and Its Effect on Vascular Endothelial Growth Factor of Sinapine of Rapeseed Meal

To investigate the antihypertensive effect and its influence on vascular endothelial growth factor of sinapine chlorate of rapeseed meal, the antihypertensive effect of sinapine chlorate on systolic blood pressure, diastolic blood pressure and mean arterial pressure was detected by arterial intubation into New Zealand white rabbits. The levels of prostacyclin (PGI2), thromboxane A2(TXA2) and endothelin-1 (ET-1) in serum were detected by enzyme linked immunosorbent assay (ELISA). The level of NO in serum was detected by nitrate reductase method. The results showed that sinapine chlorate could significantly decrease the systolic pressure, diastolic pressure and mean pressure in the range of 0.025~12.5 mg/kg. While with the dose increasing, the depressurization rate would be raised and holding time be prolonged. The results of ELISA showed that there was no significant effect on PGI2 and ET-1 in serum of experimental animals, and the level of TXA2 could be significantly reduced by the sinapine chlorate in 2.4 mg/kg and 9.6 mg/kg. Above results indicated sinapine chlorate had a significant antihypertensive effect, and the antihypertensive mechanism might be achieved by inhibiting levels of vasoconstriction factor TXA2.