杜仲愈伤组织诱导及增殖培养技术研究

杜仲是我国名贵的中药材和最有开发前景的胶源植物。通过筛选外植体、优化培养基,建立杜仲愈伤组织诱导和增殖的培养体系,利用统计学方法进行分析。结果显示:以子叶、幼叶、下胚轴为外植体均能够诱导出愈伤组织,但较佳的外植体为下胚轴,诱导率达到79.33%;以下胚轴为外植体的愈伤组织诱导基本培养基是B5,较优的激素配比为NAA 1.0 mg/L+2,4-D 2.0 mg/L;愈伤组织增殖培养的较优激素配比为6-BA 0.5mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L。建立了杜仲愈伤组织的培养方法,为通过杜仲细胞培养获得代谢产物提供了技术支持。

Study on Callus Induction and Proliferation Culture ofEucommia ulmoides

Eucommia ulmoides is one of the most valuable Chinese medicinal materials and the most promising gum source plants. In this paper, the culture system of callus induction and proliferation of Eucommia ulmoides was established by screening explants and optimizing the culture medium. The results showed that cotyledons, leaves and hypocotyls could induce callus, but the better explants were hypocotyls. The better basal medium for callus induction was B5, and the optimal phytohormones were NAA 1.0 mg/L and 2, 4-D 2.0 mg/L. The optimal phytohormone for callus proliferation culture were 6-BA 0.5 mg/L, NAA 0.5 mg/L and 2,4-D 1.0 mg/L. This paper established a method for callus culture of Eucommia ulmoides Oliv., which provided technical support for obtaining metabolites through cell culture.