基因组水平上拟南芥和水稻mrs2基因家族的比较系统发生分析

mrs2（mitochondrial RNA splicing2）基因是植物线粒体中Ⅱ类内含子自我剪接缺陷的抑制基因，同时参与了植物中镁离子的运输。本研究利用已经分离的植物的mrs2基因，鉴别出MRS2结构域，同时对拟南芥和水稻中的mrs2基因家族的成员进行了鉴定；利用这些基因编码的蛋白质序列构建了系统发生树，并进行了序列保守性分析，最后查找了相关基因的EST表达信息。结果表明：①系统发生分析表明拟南芥和水稻的mrs2基因的结构在拟南芥和水稻分离之前已经形成，并在分离之后按照物种特异性的方式进行了扩张；②MEME分析表明植物的Mrs2蛋白质具有高度保守的基序，并且在蛋白质中的排列顺序也大致相似；③mrs2基因在拟南芥和水稻中的表达有差异，但在部分表达上仍保持了一致性。

Genome-wide Comparative Phylogenetic Analysis of mrs2 |Gene Family in Arabidospsis and Rice

mrs2(mitochondrial RNA splicing 2) genes were discovered as a suppressor of splicing defects in mitochondria.They have been shown to be a transporter for the movement of Mg2 in plants.This study collected the mrs2 genes that were isolated in plants and obtained their MRS2 domains.We identified the mrs2 genes in Arabidopsis and rice.A phylogenetic tree was constructed based on the Mrs2 proteins.Analyses of the conserved sequences of these proteins were conducted;and finally the expression information of these genes was searched.The results are as follows:(1) The phylogenetic analysis indicated that the feature of this family might have been in existence before the split of Arabidopsis and rice,and species-specific expansion might have contributed to this family after the split of Arabidopsis and rice.(2) MEME analysis indicated that the motifs in the Mrs2 protein were highly conservative,and the order of these motifs in the proteins was quite similar.(3) The expressions of the mrs2 genes in Arabidopsis and rice were different,but still keep consistency in some expressions.