| 玉米幼胚和成熟胚愈伤组织分化反应性比较 |
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| 引用本文： | 曹俊梅,窦秉德,李生强,陈莉,罗玉明,白光宏. 玉米幼胚和成熟胚愈伤组织分化反应性比较[J]. 新疆农业大学学报, 2005, 28(2): 10-13 |
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| 作者姓名： | 曹俊梅 窦秉德 李生强 陈莉 罗玉明 白光宏 |
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| 作者单位： | 1. 新疆农业大学,农学院,乌鲁木齐,830052
2. 淮阴师范学院生物系,江苏,淮安,223300 |
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| 基金项目： | 江苏省教育厅高新技术项目(JH03-003) |
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| 摘 要： | 以鲜食玉米为主的13种基因型的成熟胚和幼胚为材料，对它们的愈伤组织诱导．继代培养和分化进行了系统研究。结果表明：玉米的成熟胚和幼胚愈伤诱导率相关，但两种胚诱导出的愈伤组织状态明显不同．幼胚诱导出的愈伤组织状态普遍比成熟胚的好；两种胚的愈伤分化率差异明显，成熟胚愈伤组织最高分化率为2．5％,幼胚愈伤组织最高分化率可达83．3％。
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| 关 键 词： | 玉米 成熟胚 幼胚 组织培养 愈伤组织 组织分化 |
| 文章编号： | 1007-8614(2005)02-0010-04 |
| 修稿时间： | 2005-04-23 |
A comparison on tissue culture ability between maize nature embryo and immature embryo |
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| CAO Jun-mei,DOU Bing-de,LI Sheng-qiang,CHEN Li,LUO Yu-ming,BAI Guang-hong. A comparison on tissue culture ability between maize nature embryo and immature embryo[J]. Journal of Xinjiang Agricultural University, 2005, 28(2): 10-13 |
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| Authors: | CAO Jun-mei DOU Bing-de LI Sheng-qiang CHEN Li LUO Yu-ming BAI Guang-hong |
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| Affiliation: | CAO Jun-mei~1,DOU Bing-de~2,LI Sheng-qiang~1,CHEN Li~1,LUO Yu-ming~2,BAI Guang-hong~1 |
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| Abstract: | This paper deals with studies on initiation, maintenance and differentiation of callus from mature embryo and immature embryo of thirteen genotypes in corn. The results showed that the callus induced frequency were correlated between mature embryo and immature embryo,but the callus quality was obviously different. Calli obtained from immature embryo were generally batter than those of mature embryo. The differentiation frequency was widely different between the two kinds of callus, only 2.5% differentiation frequency could be obtained on mature embryo callus, and the differentiation frequency on immature embryo callus could be up to 83.3%. |
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| Keywords: | maize mature embryo immature embryo tissue culture |
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