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| 根癌农杆菌介导的叶用莴苣基因转化系统的建立 | |
| 引用本文： | 罗淑萍,张智俊,秋田求.根癌农杆菌介导的叶用莴苣基因转化系统的建立[J].新疆农业大学学报,2005,28(1):30-33. |
| 作者姓名： | 罗淑萍 张智俊 秋田求 |
| 作者单位： | 1. 新疆农业大学,农学院,乌鲁木齐,830052 2. 南京大学,生命科学学院,南京,210093 3. 日本近畿大学,生物理工学部,和歌山,6496493 |
| 摘 要： | 以叶用莴苣微茎尖为试材，在含有BA和NAA的MS液体培养基上旋转培养，诱导出大量愈伤组织；进一步分化培养，获得大量不定芽并生根；将愈伤组织碎块与根癌农杆菌共培养，对经卡那霉素筛选后所得到的再生植株进行X-Gluc染色，蓝色反应阳性率为80％，表明建立了叶用莴苣愈伤组织基因转化系统。 |
| 关 键 词： | 根癌农杆菌 叶用莴苣 基因转化 愈伤组织 组织培养 植株再生 |
| 文章编号： | 1007-8614(2005)01-0030-04 |
| 修稿时间： | 2005年1月10日 |
Establishment of Agrobacterium tumefaciens mediated gene transformation system of leaf lettuce |
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| LUO Shu-ping,ZHANG Zhi-jun,Yoshimoto Ohta.Establishment of Agrobacterium tumefaciens mediated gene transformation system of leaf lettuce[J].Journal of Xinjiang Agricultural University,2005,28(1):30-33. | |
| Authors: | LUO Shu-ping ZHANG Zhi-jun Yoshimoto Ohta |
| Institution: | LUO Shu-ping~1,ZHANG Zhi-jun~2,Yoshimoto Ohta~3 |
| Abstract: | Primordium-like tissue was established from the apical meristem under rotation culture on MS medium with BA and NAA. Adventitious shoots with roots were efficiently induced from the tissue. After the shivered primordium-like tissue were co-cultured with Agrobacterium tumefaciens and the regenerated plants were selected by kanamycin, the regenerated plants showed 80% of positive reaction when their leaf slices dyed with X-Gluc solution. The results showed that the gene transformation system of primordium-like tissue of leaf lettuce was successfully established. |
| Keywords: | Lactuca sativa L Agrobacterium tumefaciens gene transformation system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |