| 猪圆环病毒Ⅱ型ORF1基因的克隆与原核表达 |
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| 引用本文： | 曾朔,吴星星,冉多良,陈丽娟,胡雯婷.猪圆环病毒Ⅱ型ORF1基因的克隆与原核表达[J].新疆农业大学学报,2011,34(4):307-310. |
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| 作者姓名： | 曾朔 吴星星 冉多良 陈丽娟 胡雯婷 |
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| 作者单位： | 新疆农业大学动物医学学院,乌鲁木齐,830052 |
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| 基金项目： | 新疆维吾尔自治区重点产业紧缺人才专业大学生创新项目(jqrcp2201028) |
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| 摘 要： | 根据GenBank中猪圆环病毒Ⅱ型(PCV2) ORF1基因序列,设计合成了一对引物,扩增出PCV2新疆株ORF1基因片段.将扩增出的ORF1基因(945 bp)插入到pMD18-T载体中,筛选获得重组质粒,命名为pMD18-ORF1.将ORFI酶切产物亚克隆到原核表达载体pET-32a中,获得重组质粒,命名为pET-...
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| 关 键 词： | PCV ORF1 克隆 原核表达 |
Cloning and Prokaryotic Expression of ORF1 Gene of Porcine Circovirus Type 2 |
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| ZENG Shuo,WU Xing-xing,RAN Duo-liang,CHEN Li-juan,HU Wen-ting.Cloning and Prokaryotic Expression of ORF1 Gene of Porcine Circovirus Type 2[J].Journal of Xinjiang Agricultural University,2011,34(4):307-310. |
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| Authors: | ZENG Shuo WU Xing-xing RAN Duo-liang CHEN Li-juan HU Wen-ting |
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| Institution: | ZENG Shuo,WU Xing-xing,RAN Duo-liang,CHEN Li-juan,HU Wen-ting(College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,China) |
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| Abstract: | This study was conducted to design,a pair of primer according to PCV2 ORF1 gene sequence in the Genbank.The ORF1 gene was amplified from suspected PMWS sample via polymerase chain reaction(PCR),and the gene was cloned into pMD18-T vector.Then the ORF1 gene was subcloned into prokaryotic expressing vector pET-32a,the recombinant plasmid was named pET-32a-ORF1,and was induced by IPTG.The results of SDS-PAGE and Western-blotting indicated that the ORF1 gene was expressed in a high level,and the recombinant fus... |
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| Keywords: | PCV2 ORFl gene cloning prokaryotic expression |
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