| 鸡传染性支气管炎病毒XJ株N基因的克隆表达及抗原性初步鉴定 |
| |
| 引用本文： | 齐晓亮,夏俊,成进,陆桂丽,沙依兰古丽,汪萍.鸡传染性支气管炎病毒XJ株N基因的克隆表达及抗原性初步鉴定[J].新疆农业大学学报,2010,33(2):142-145. |
| |
| 作者姓名： | 齐晓亮 夏俊 成进 陆桂丽 沙依兰古丽 汪萍 |
| |
| 作者单位： | 1. 新疆畜牧科学院,兽医研究所,乌鲁木齐,830000;新疆农业大学,动物医学学院,乌鲁木齐,830052
2. 新疆畜牧科学院,兽医研究所,乌鲁木齐,830000 |
| |
| 基金项目： | 农业部科教司农业公益性行业科研专项 |
| |
| 摘 要： | 根据发表的鸡传染性支气管炎病毒N基因序列设计一对引物，经RT—PCR扩增得到1230bpDNA片段，将其克隆到表达载体pET-28a（＋）中，构建重组质粒pET—N，经测序鉴定正确后，将其转化感受态细胞E．coli BL21（DE3），并进行IPTG诱导表达，结果表明，重组菌可表达分子质量为50kD的融合蛋白。Western-blotting结果显示，该重组蛋白能与IBV阳性血清发生特异性的免疫印迹反应，表明该重组蛋白具有良好的免疫原性。
|
| 关 键 词： | 鸡传染性支气管炎病毒 N基因 克隆表达 免疫印迹 |
Preliminary Study on Cloning and Expression of Nucleocapsid Protein Gene of Avian Infectious Bronchitis Virus XJ Strain and of Its Antigenicity |
| |
| QI Xiao-liang,XIA Jun,CHENG Jin,LU Gui-li,Shayilanguli,WANG Ping.Preliminary Study on Cloning and Expression of Nucleocapsid Protein Gene of Avian Infectious Bronchitis Virus XJ Strain and of Its Antigenicity[J].Journal of Xinjiang Agricultural University,2010,33(2):142-145. |
| |
| Authors: | QI Xiao-liang XIA Jun CHENG Jin LU Gui-li Shayilanguli WANG Ping |
| |
| Institution: | QI Xiao-liang1,2,XIA Jun1,CHENG Jin1,LU Gui-li1,Shayilanguli1,WANG Ping1(1.Institute of Veterinary Science,Xinjiang Academy of Animal Science,Urumqi 830000,China,2.College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,China) |
| |
| Abstract: | A 1 230 bp nucleocapsid(N) protein gene of IBV was amplified by RT-PCR using a pair of primers designed based on the N gene sequence.The product was cloned into the expression plasmid vector pET-28a(+)to construct a recombinant pET-N.After sequencing,the recombinant was transformed into Escherichia E.coli(DE3) competent cells.The transformed bacteria were induced by IPTG to produce a recombinant protein of 50 kD of molecular weight.The Western-blotting result showed that the protein could react with the pos... |
| |
| Keywords: | avian infectious bronchitis virus N gene cloning and expression Western-blotting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
