电磁场对鲫血清4种生化指标的影响研究

为研究电磁场对鱼类生理的影响,试验中将体质量为(103±2)g的40尾鲫Carassius auratus幼鱼分组放置在相同水温(10℃±1℃)、不同电磁场强度(0、0.5、1.0、1.5、2.0 mT的恒定磁场)下培养,并对每组鱼血清中的碱性磷酸酶(AKP)、乙酰胆碱酯酶(AChE)、溶菌酶(LZM)活性和蛋白含量进行测定,试验周期为10 d,电磁场环境由亥姆霍兹线圈产生。结果表明:从试验第2天开始,电磁场试验组AKP活性明显低于对照组(P0.05);电磁场试验组AChE活性在2~10 d内逐渐减弱,并最终下降至初始活性的85.98%~95.47%;试验结束时,电磁场试验组LZM活性显著低于对照组(P0.05),蛋白含量较初始水平降低了24.61%。研究表明,电磁场对鲫血清4种生化指标的影响基本为抑制作用,说明多数外界环境的变化对鱼体本身会造成不利影响。

Effects of electromagnetic field on four biochemical indices of blood in crucian carp

The activities of alkaline phosphatase (AKP),acetylcholinesterase (AChE), and lysozyme (LZM) in the blood were analyzed in 40 individual crucian carp juveniles with body weight of(103±2) g exposed to electro-magnetic field at a intensity of 0,0.5,1.0,1.5,2.0 mT constant magnetic field generated by the Helmholtz coil at same water temperature of(10±1) ℃ for 10 days to study the influence of electromagnetic fields on fish physiology. The results showed that there was significantly lower activity of AKP in the treated fish than that in the control group from the second day of the experiment(P<0.05). From second day to the tenth day of the experiment, the fish in treated group had gradually declined activity of AChE and finally declined to 85.98%-95.47%of the initial activity. The activity of lysozyme in the fish was found to be significantly lower in the treatment group than that in the control group (P<0.05),the muscular protein level being reduced by 24.61% compared to the initial level at end of the experiment,indicating that the electromagnetic field leads to inhibit blood biochemical parameters and thus to dis-advantageous effect for fishes.