应用16S rRNA基因文库技术分析3种生物填料上生物膜的细菌群落组成

为研究生物填料上生物膜的细菌群落组成及其在循环水养殖系统中的应用,选择3种生物填料构建生物滤池,采用细菌16S rRNA基因片段扩增和Illumina高通量测序法,分析了循环水养殖系统中填料上生物膜和对应生物滤池水体中的细菌群落种类组成。结果表明:3种生物填料均有富集微生物生长的作用,生物填料上生物膜的细菌种类和多样性大于对应的生物滤池水体;在门分类水平,3个生物滤池水体中的优势菌相同,均为厚壁菌门,3种生物填料上生物膜的优势菌不尽相同,聚乙烯小球生物膜的优势菌为厚壁菌门,陶瓷环和弹性毛刷生物膜的优势菌为变形菌门;弹性毛刷生物膜的细菌种类最多、多样性指数最高,由变形菌门的26个属细菌组成,聚乙烯小球生物膜的细菌种类组成最少、多样性指数最低,由厚壁菌门的23个属组成,细菌丰度指数最低。

Analysis of bacterial community compositions in three biological filter media by 16 S rRNA gene library

The present study was aimed to analyze the composition of bacteria community and to evaluate the appli-cations of biological filter media in recirculating aquaculture system. Bio-filters were constructed by three types of media and the bacterial community compositions of bio-film as well as water samples were studied using 16 S rRNA gene amplification and Illumina high-throughput sequencing platform. The results showed that the three media all enriched the bacterial abundance and diversity, and that there were more bacterial species and higher biodiversity in the three media than those in the water samples. The predominant bacterial phylum was the same Firmicutes in all water samples of the three bio-filters. On the three media, however, there were different predominant bacteria in bio-film, including Firmicutes in polyethylene ball medium and Proteobacteria in submerged ceramics medium and elastic brush medium. In terms of bacterial species and diversity, the maximal value (26 genera) was observed in elastic brush medium. However, 23 genera, the minimal species composition, was detected in polyethylene ball medium.