红鳍东方鲀抗苗勒氏管激素(AMH)基因在不同发育时期的组织表达

为研究抗苗勒氏管激素(AMH)基因在红鳍东方鲀Takifugu rubripes各组织和不同发育时期的表达情况以及在性别分化和性腺发育等过程中的作用,利用荧光实时定量PCR(qRT-PCR)技术对红鳍东方鲀成鱼的肾脏、脑、心脏、脾脏、肝脏、精巢和卵巢等组织进行了amh基因的表达分析以及不同发育时期(23、30、40、60、80日龄及2龄和3龄鱼)红鳍东方鲀个体水平上amh基因的表达分析。结果表明:红鳍东方鲀3龄成鱼脾脏、肾脏和肝脏中的amh基因表达量极显著高于其他组织(P0.01),在精巢和心脏中的表达量极显著高于卵巢和脑(P0.01),其中脾脏中表达量最高,脑中表达量最低;红鳍东方鲀23日龄、30日龄、2龄和3龄鱼中,雄鱼amh基因的表达量极显著高于同期雌鱼(P0.01),而60日龄和80日龄时雌鱼表达量显著高于雄鱼(P0.05),40日龄时雌、雄鱼的表达量无显著性差异(P0.05),3龄时雌、雄个体性腺中amh基因的表达量均显著高于2龄鱼(P0.05);雄性幼鱼amh基因的表达量从23~30日龄呈增长趋势,且在30日龄时达到最高,随后逐渐降低,而雌鱼整体amh基因的表达量也呈现先升高后降低的趋势,80日龄时雌鱼amh基因表达量最高。研究表明,amh基因在红鳍东方鲀的性腺发育和性别分化过程中发挥着一定的作用。

Expression of anti-Müllerian hormone (AMH) gene in different tissues and developmental phases in redfin puffer Takifugu rubripes

In this study, expressions of anti- Müllerian hormone ( AMH) in kidney, brain, heart,spleen, liver, testis and ovary were investigated in different developmental stages in redfin puffer Takifugu rubripes including 23 , 30, 40, 60, and 80 days old, and 2, and 3 years old individuals by fluorescence quantitative Real-time Polymer-ase Chain Reaction ( qRT-PCR ) . The results showed that there was very significantly higher expression of amh gene in spleen, kidney and liver than that in other organs (P<0. 01),very significantly higher in testis than that in ovary and brain, the maximal in spleen, and the minimal in brain(P<0. 01). There was very significantly higher expression level of amh gene in male redfin puffer with 23 and 30 days old, and 2 and 3 years old than that in fe-male individuals (P<0. 01). In the 60 days and 80 days old individuals, however, the female had significantly higher expression level of amh gene than the male did ( P<0. 05 ) . No significant difference in expression level of amh gene was observed between males and females in 40 days old individuals ( P>0. 05 ) . The 3 years old redfin puffer showed significantly higher expression of amh gene than the 2 years old one did ( P<0. 05 ) . The amh gene expression level in males was increased from 23 days old to 30 days old, the maximal in the 30 days old indivduals, and then decreased in later stages, while the amh gene gene expression level in females was increased from 23 days old to 80 days old and subsequently was decreased in later stages. The findings indicate that amh gene may play an important role in gonadal development and sex differentiation in redfin puffer.