| 香蕉细菌性枯萎病菌实时荧光PCR检测方法的建立 |
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| 引用本文： | 漆艳香,谢艺贤,张辉强,朱水芳,肖启明,廖晓兰.香蕉细菌性枯萎病菌实时荧光PCR检测方法的建立[J].华南热带农业大学学报,2005,11(1):1-5. |
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| 作者姓名： | 漆艳香 谢艺贤 张辉强 朱水芳 肖启明 廖晓兰 |
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| 作者单位： | [1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]国家出入境检验检疫局植检所,北京100029 [3]湖南农业大学生物安全科技学院,长沙410128 |
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| 基金项目： | 国家质检总局植物病原检测专项(Z2000-3-182) |
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| 摘 要： | 根据香蕉细菌性枯萎病菌与其它细菌菌株16SrDNA序列差异，设计并合成一对引物和一条具有稳定点突变特异性探针，建立了对香蕉细菌性枯萎病菌的实时荧光PCR检测方法。除烟草青枯病菌和马铃薯青枯病菌外，还对其它属细菌菌株进行了荧光PCR检测。结果表明，只有香蕉细菌性枯萎病菌产生荧光，其它细菌都没有荧光产生。从而证明此方法特异性强，灵敏度高，适合病害的快速诊断和口岸检验检疫应用。
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| 关 键 词： | 香蕉 细菌性枯萎病菌 实时荧光PCR检测方法 TaqMan探针 细菌基因组 |
Detection of Ralstonia Solanacearum by Real-time Fluorescent PCR |
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| Qi yanxiang,Xie yixian,Zhang Huiqiang,Zhu Shuifang,Xiao Qiming,Liao Xiaolan.Detection of Ralstonia Solanacearum by Real-time Fluorescent PCR[J].Journal of South China University of Tropical Agriculture,2005,11(1):1-5. |
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| Authors: | Qi yanxiang Xie yixian Zhang Huiqiang Zhu Shuifang Xiao Qiming Liao Xiaolan |
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| Institution: | Qi yanxiang1 Xie yixian1 Zhang Huiqiang1 Zhu Shuifang2 Xiao Qiming3 Liao Xiaolan3 |
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| Abstract: | A subgroup-specific probe was designed on the basis of 16s rDNA of ralstonia solancearum race 2 and used to detect other species of bacteria besides Ralstonia solancearum races 1 and 3. The results show that no signal was detected in other species than Ralstonia solancearum race. Few contaminations will occur because the whole detection process ends in the contained tubes, thus proving the method to be specific, highly sensitive and applicable to the immediate detection of Ralstonia solanacearum race and to the port quarantine. |
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| Keywords: | banana ralstonia solancearum TaqMan probe real-time fluorescent PCR detection |
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