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紫外分光光度法测定蔬菜硝酸盐含量
引用本文:罗雪华,蔡秀娟.紫外分光光度法测定蔬菜硝酸盐含量[J].华南热带农业大学学报,2004,10(1):13-16.
作者姓名:罗雪华  蔡秀娟
作者单位:中国热带农业科学院橡胶所 海南儋州571737 (罗雪华),中国热带农业科学院橡胶所 海南儋州571737(蔡秀娟)
摘    要:以硝酸盐含量差别很大的两种蔬菜(苦瓜和芥菜)为材料,采用硼砂、亚铁氰化钾、乙酸锌、活性碳粉制备待测液,有效地去除了有机物质的干扰。进而用紫外光度法直接测定待测液中的N03。苦瓜和芥菜N03测定结果的变异系数分别为0.9%和1.3%,回收率分别为97.1%、105.7%,平均回收率101.4%。

关 键 词:紫外分光光度法  测定技术  蔬菜  硝酸盐  含量

Determination of Nitrate Content in Vegetables by UV-Spectrophotometric Method
Luo Xuehua,Cai Xiujuan.Determination of Nitrate Content in Vegetables by UV-Spectrophotometric Method[J].Journal of South China University of Tropical Agriculture,2004,10(1):13-16.
Authors:Luo Xuehua  Cai Xiujuan
Abstract:Balsam pear and mustard, which differ greatly in the content of nitrate, were used as test materials, hot water, borax, potassium ferrocyanide, zinc acetate, and activated carbon powder were adopted to make extract, and then UV-spectrophotometric method was used to determine the nitrate content in the extract directly. According to the determination results, the variation of nitrate in balsam pear and mustard were 0.9% and 1.3% respectively, the recovery were 71.1% and 105.7% respectively, and the average recovery was 104.1%. The test shows that UV-spectrophotometric method is simple, rapid and credible, thus applicable to the analysis of N03 in vegetables.
Keywords:UV-spectrophotometric method    vegetables    nitrate
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