| 茶树基因组DNA提取方法的研究 |
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| 引用本文： | 游小妹,林郑和,陈常颂,陈荣冰.茶树基因组DNA提取方法的研究[J].江西农业学报,2008,20(2):34-37. |
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| 作者姓名： | 游小妹 林郑和 陈常颂 陈荣冰 |
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| 作者单位： | 福建省农业科学院,茶叶研究所,福建,福安,355015 |
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| 摘 要： | 对传统的CTAB与SDS法进行改良与简化，从茶树嫩梢部位提取高质量的基因组DNA。结果表明，用该法提取的DNA的OD260／OD280在1．8～1．9之间，电泳与PCR都能得到清晰的图谱。
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| 关 键 词： | 茶树 DNA 提取方法 电泳 PCR |
| 文章编号： | 1001-8581(2008)02-0034-04 |
| 修稿时间： | 2007年11月13 |
Research on Methods for Genomic DNA Extraction from Tea Plant |
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| YOU Xiao-mei,LIN Zheng-he,CHEN Chang-song,CHEN Rong-bing.Research on Methods for Genomic DNA Extraction from Tea Plant[J].Acta Agriculturae Jiangxi,2008,20(2):34-37. |
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| Authors: | YOU Xiao-mei LIN Zheng-he CHEN Chang-song CHEN Rong-bing |
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| Institution: | YOU Xiao-mei, LIN Zheng-he, CHEN Chang-song, CHEN Rong-bing(Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu＇an 355015,China) |
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| Abstract: | High-quality genomic DNA from leaves of tea plant was extracted by improved CTAB and SDS method.Results showed that the values of OD260/OD280 of the DNA samples obtained with the improved method were between 1.8 and 1.9,which could be determined by electrophoresis and PCR. |
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| Keywords: | Tea plant DNA Extraction method Electrophoresis PCR |
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