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| 牛朊蛋白在CHO/dhfr-细胞中的稳定表达 | |
| 引用本文： | 丁耀忠,张杰,刘永生,陈豪泰,曾巧英,马丽娜,马艳平,杨生海,殷宏.牛朊蛋白在CHO/dhfr-细胞中的稳定表达[J].江西农业学报,2009,21(4). |
| 作者姓名： | 丁耀忠 张杰 刘永生 陈豪泰 曾巧英 马丽娜 马艳平 杨生海 殷宏 |
| 作者单位： | 1. 甘肃农业大学,甘肃,兰州,730070;中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室、农业部,畜禽病毒学重点开放实验室、农业部,兽医公共卫生重点开放实验室,甘肃,兰州,730046 2. 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室、农业部,畜禽病毒学重点开放实验室、农业部,兽医公共卫生重点开放实验室,甘肃,兰州,730046 3. 甘肃农业大学,甘肃,兰州,730070 |
| 摘 要： | 从T载体上得到的目的基因和dhfr共扩增基因真核表达载体构建了pCI-PrP102。纯化后的重组质粒通过脂质体转染CHO/dhfr-细胞,经MTX加压筛选获得稳定表达的细胞株,间接免疫荧光试验检测到目的蛋白的表达。 |
| 关 键 词： | 牛朊蛋白 二氢叶酸还原酶缺陷型CHO细胞 稳定表达 |
Stable Expression of Bovine Prion Protein in CHO/dhfr-cells |
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| DING Yao-zhong,ZHANG Jie,LIU Yong-sheng,CHEN Hao-tai,ZENG Qiao-ying,MA Li-na,MA Yan-ping,YANG Sheng-hai,YING Hong.Stable Expression of Bovine Prion Protein in CHO/dhfr-cells[J].Acta Agriculturae Jiangxi,2009,21(4). | |
| Authors: | DING Yao-zhong ZHANG Jie LIU Yong-sheng CHEN Hao-tai ZENG Qiao-ying MA Li-na MA Yan-ping YANG Sheng-hai YING Hong |
| Institution: | 1.Gansu Agricultural University;Lanzhou 730070;China;2.Key Laboratory of Veterinary Public Health;Ministry of Agriculture;Key Laboratory of Animal Virology;State Key Laboratory of Veterinary Etiological Biology;Lanzhou Veterinary Research Institute;Chinese Academy of Agricultural Sciences;Lanzhou 730046;China |
| Abstract: | The target genes from T-vector were connected with dhfr co-amplified gene of eukaryotic vector and constructed pCI-PrP102.Purified recombinant plasmids were transferred into CHO/dhfr-cell through liposome,and obtained the stable expression of the cell strains after MTX screening,and indirect immunity fluorescence analysis detected the expression of the goal protein. |
| Keywords: | Bovine prion protein CHO/dhfr-cell Stable expression |
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