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用双波长分光光度法测定鲜食玉米中直链淀粉和支链淀粉含量
引用本文:林美娟,宋江峰,李大婧,刘春泉.用双波长分光光度法测定鲜食玉米中直链淀粉和支链淀粉含量[J].江西农业学报,2010,22(12).
作者姓名:林美娟  宋江峰  李大婧  刘春泉
作者单位:1. 南京师范大学,金陵女子学院,江苏,南京,210097;江苏省农业科学院,农产品加工研究所,江苏,南京,210014;国家农业科技华东,江苏,创新中心,农产品加工工程技术研究中心,江苏,南京,210014
2. 江苏省农业科学院,农产品加工研究所,江苏,南京,210014;国家农业科技华东,江苏,创新中心,农产品加工工程技术研究中心,江苏,南京,210014
基金项目:江苏省农业科技自主创新资金项目
摘    要:以碘为显色剂,应用双波长分光光度法测定了鲜食玉米中直链淀粉和支链淀粉的含量。根据碘-直链淀粉和碘-支链淀粉复合物的吸收光谱,选择直链淀粉的测定波长为629 nm和463 nm,支链淀粉的测定波长为553 nm和738 nm,并依据回归方程求出直链淀粉和支链淀粉的含量。直链淀粉的含量在10~60 mg/L、支链淀粉在60~200 mg/L范围内符合比耳定律。用双波长法测定不同品种鲜食玉米中直链淀粉和支链淀粉含量取得了良好的效果。

关 键 词:双波长分光光度法  鲜食玉米  直链淀粉含量  支链淀粉含量  测定

Determination of Amylose and Amylopectin Content in Fresh Corn by Dual-wavelength Spectrophotometry
LIN Mei-juan,SONG Jiang-feng,LI Da-jing,LIU Chun-quan.Determination of Amylose and Amylopectin Content in Fresh Corn by Dual-wavelength Spectrophotometry[J].Acta Agriculturae Jiangxi,2010,22(12).
Authors:LIN Mei-juan  SONG Jiang-feng  LI Da-jing  LIU Chun-quan
Abstract:Dual-wavelength spectrophotometry was applied to the determination of amylose and amylopectin in the fresh corn through using iodine as coloring agent.According to the absorptive spectra of iodine-amylose and iodine-amylopectin,the wavelength of 629 nm and 463 nm were selected for the determination of amylose,wavelength of 553 nm and 738 nm were selected as the measuring wavelength for amylopectin.The content of amylose and amylopectin could be calculated based on the two regressive equations.Beer's law was obeyed in 10~60 mg/L for amylose and 60~200 mg/L for amylopectin.The results of amylose and amylopectin content in the fresh corn determined by the method were satisfactory.
Keywords:Dual-wavelength spectrophotometry  Fresh corn  Amylose  Amylopectin  Determination
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