| 耧斗菜愈伤组织和不定芽诱导的初步研究 |
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| 引用本文： | 朱蕊蕊,杨姗姗,明晓,高亦珂.耧斗菜愈伤组织和不定芽诱导的初步研究[J].江西农业学报,2009,21(7):81-82,86. |
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| 作者姓名： | 朱蕊蕊 杨姗姗 明晓 高亦珂 |
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| 作者单位： | 北京林业大学,园林学院,北京,100083 |
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| 基金项目： | 北京林业大学大学生科技创新计划 |
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| 摘 要： | 以耧斗菜(Aquilegia vulgaris)为实验材料,研究了不同外植体以及生长调节物质对其愈伤组织和不定芽再生的影响,实验结果表明:叶片作为外植体,培养基为MS+2.0 mg/L NAA+2.0 mg/L6-BA时,较适宜于诱导愈伤组织,诱导率达到92.6%;诱导不定芽的较适宜培养基为MS+0.1 mg/L NAA+1.0 mg/L6-BA。
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| 关 键 词： | 耧斗菜 愈伤组织 不定芽 诱导 |
Research on Regeneration of Callus and Adventitious Bud of Aquilegia vulgaris |
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| ZHU Rui-rui,YANG Shan-shan,MING Xiao,GAO Yi-ke.Research on Regeneration of Callus and Adventitious Bud of Aquilegia vulgaris[J].Acta Agriculturae Jiangxi,2009,21(7):81-82,86. |
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| Authors: | ZHU Rui-rui YANG Shan-shan MING Xiao GAO Yi-ke |
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| Institution: | College of Horticulture and Gardens;Beijing Forestry University;Beijing 100083;China |
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| Abstract: | The effects of different explants and growth regulating substances to the regeneration of callus and adventitious bud of Aquilegia vulgaris were researched.The results showed that when leaf was chosen as the explant and the culture medium was MS+2.0 mg/L NAA +2.0 mg/L 6-BA,the callus could be easily induced,the inducement rate reached to 92.6%;MS+0.1mg/L NAA +1.0mg/L 6-BA was suitable for the inducement of adventitious bud. |
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| Keywords: | Aquilegia vulgaris Callus Adventitious bud Inducement |
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