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| 简易快速从微量羊血中提取高质量基因组的方法 | |
| 引用本文： | 陈冰,王富强,刘德稳,高腾云.简易快速从微量羊血中提取高质量基因组的方法[J].江西农业学报,2008,20(1):103-104,107. |
| 作者姓名： | 陈冰 王富强 刘德稳 高腾云 |
| 作者单位： | 1. 河南农业大学,牧医工程学院,河南郑州 450002 2. 商丘韦斯迪生物技术公司,河南商丘,476000 3. 山东德州学院,山东德州,253023 |
| 摘 要： | 以碘化钾法为基础，建立了一种简单快速从微量羊血中提取高质量基因组DNA的改进方法。该方法提取的基因组DNA经0．6％琼脂糖凝胶电泳检测，DNA带整齐，无拖尾。OD260／OD280为1．81±O．13，浓度为（234±36．4）μg／mL，PCR扩增得到了满意的扩增效果。 |
| 关 键 词： | 羊血 基因组DNA 提取 PCR |
| 文章编号： | 1001-8581（2008）01-0103-02 |
| 收稿时间： | 2007-11-05 |
| 修稿时间： | 2007年11月5日 |
Simple and Quick Method for Extracting High-quality Genomic from Small Volume Goat Blood |
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| CHEN Bing,WANG Fu-qiang,LIU De-wen,GAO Teng-yun.Simple and Quick Method for Extracting High-quality Genomic from Small Volume Goat Blood[J].Acta Agriculturae Jiangxi,2008,20(1):103-104,107. | |
| Authors: | CHEN Bing WANG Fu-qiang LIU De-wen GAO Teng-yun |
| Institution: | CHEN Bing , WANG Fu - qiang, LIU De - wen, GAO Teng - yun (1. College of Animal Husbandry and Veterinary ,Henan Agricultural University,Zhengzhou 450002,China; 2. Visidy Bio - tech Company of Shangqiu City, Shangqiu 476000, China ;3. Dezhou University, Dezhou 253023, China) |
| Abstract: | An improved simple and quick method for extracting high-quality genomic DNA from small volume goat blood was established based on Potassium Iodide method.The genomic DNA was detected by 0.6% agar sugar gel electrophoresis,the result revealed the major bands of the genomic DNA were clear without the phenomenon of long tail.The OD260/OD280of DNA was 1.81±0.13 and the concentration was(234±36.4) μg/mL.The gained genomic DNA had good results of PCR amplification. |
| Keywords: | Goat blood Genomic DNA Extract PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |