| 鹅细小病毒PCR诊断方法的初步建立 |
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| 引用本文： | 刘宇卓,李银,魏雪涛,张敬峰.鹅细小病毒PCR诊断方法的初步建立[J].江西农业学报,2011,23(3). |
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| 作者姓名： | 刘宇卓 李银 魏雪涛 张敬峰 |
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| 作者单位： | 江苏省农业科学院兽医研究所、农业部,动物疫病诊断与免疫重点开放实验室、国家兽用生物制品工程技术研究中心,江苏,南京,210014 |
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| 基金项目： | 江苏省农业科技自主创新资金项目(CX |
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| 摘 要： | 根据GeneBank上已发表的鹅细小病毒(GPV)基因序列,设计合成1对引物,以GPV阳性毒株鹅胚尿囊液及攻毒后死亡的雏鹅组织,提取DNA并进行PCR,结果产物扩增出的片段与预期片段440 bp大小相符,测定序列与GeneBank上GPV B株同源性达99%。试验结果显示用该方法可以特异性地诊断出GPV,比琼脂扩散试验(AGP)敏感性高,可检测最低浓度为102.5GELD50/0.2mL;检测攻毒后死亡的雏鹅组织,在脑、肝、肺、肾、肠等组织中均有GPV的存在,可以用于临床可疑病毒的分离鉴定。所建立的PCR方法具有高度的特异性、敏感性和可重复性。
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| 关 键 词： | 鹅 鹅细小病毒 PCR 琼脂扩散试验 |
Preliminary Establishment of PCR Method for Detection of Goose Parvovirus |
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| LIU Yu-zhuo,LI Yin,WEI Xue-tao,ZHANG Jing-feng.Preliminary Establishment of PCR Method for Detection of Goose Parvovirus[J].Acta Agriculturae Jiangxi,2011,23(3). |
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| Authors: | LIU Yu-zhuo LI Yin WEI Xue-tao ZHANG Jing-feng |
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| Institution: | LIU Yu-zhuo; LI Yin; WEI Xue-tao; ZHANG Jing-feng(Institute of Veterinary Medicine; Jiangsu Academy of Agricultural Sciences; Key Laboratory of Animal Diseases Diagnosis and Immunology; Ministry of Agriculture; National Center for Engineering Research of Veterinary Bio-products; Nanjing 210014; China); |
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| Abstract: | DNA was extracted from the tissue of the dead goose caused by the attack of Goose Parvovirus(GPV) as well as from allantoic liquid GPV,a pair of primers were designed according to the sequence of GPV published in GenBank,and an expected 440 bp segment was amplified by PCR.The sequencing result showed that the homology was 99% in comparison with the reference sequence of GPV strain B which was published in GenBank.This test showed that this PCR method could specially detect GPV and was more sensitive in dete... |
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| Keywords: | PCR |
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