| 蝴蝶兰花梗诱导培养优化技术研究 |
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| 引用本文： | 谢启鑫,黄虹,孙芳芳,宋小民,黄冬华,李宝光.蝴蝶兰花梗诱导培养优化技术研究[J].江西农业学报,2011,23(3):32-33,39. |
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| 作者姓名： | 谢启鑫 黄虹 孙芳芳 宋小民 黄冬华 李宝光 |
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| 作者单位： | 1. 江西省农业科学院,江西南昌,330200
2. 江苏省苏州市蔬菜研究所,江苏苏州,215008 |
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| 摘 要： | 以蝴蝶兰花梗为外植体,探讨了不同类型的培养基以及在培养基中添加激素对花梗诱导、丛生芽增殖和成苗的影响。结果表明:培养基1/2MS+BA 3 mg/L+NAA 0.2 mg/L适合花梗诱导,诱导率达90%以上;丛生芽增殖培养基以1/2MS+BA 5 mg/L+NAA 0.2 g/L的综合效果好,培养20～30 d后的增殖系数达到3.1;适合生根的培养基为MS(1/2N)+IBA 0.1mg/L+NAA 0.2 mg/L。
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| 关 键 词： | 蝴蝶兰 花梗诱导 组织培养 优化 |
Optimization of Tissue Culture Technology of Pedicel in Phalaenopsis amabilis |
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| XIE Qi-xin,HUANG Hong,SUN Fang-fang,SONG Xiao-min,HUANG Dong-hua,LI Bao-guang.Optimization of Tissue Culture Technology of Pedicel in Phalaenopsis amabilis[J].Acta Agriculturae Jiangxi,2011,23(3):32-33,39. |
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| Authors: | XIE Qi-xin HUANG Hong SUN Fang-fang SONG Xiao-min HUANG Dong-hua LI Bao-guang |
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| Institution: | 1(1.Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2.Suzhou Vegetable Research Institute of Jiangsu Province,Suzhou 215008,China) |
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| Abstract: | Taking the pedicel of Phalaenopsis amabilis as the explant,this paper studied the effects of different media and different hormone combinations on the propagation of buds and formation of seedlings.The results showed that the suitable medium for the inducement of pedicel of Phalaenopsis amabilis was 1/2MS+BA 3 mg/L+NAA 0.2 mg/L,and the inducement rate was above 90%.The suitable medium for the propagation of adventitious buds was 1/2MS+BA 5 mg/L+NAA 0.2 g/L,and the propagation coefficient was 3.1 after 20～30... |
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| Keywords: | Phalaenopsis amabilis Inducement of pedicel Tissue culture Optimization |
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