| 亲环素基因在烟草不同器官及叶片不同发育时期的表达规律研究 |
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| 引用本文： | 许俐,王红丽,刘国顺,杨惠娟.亲环素基因在烟草不同器官及叶片不同发育时期的表达规律研究[J].江西农业学报,2013(11):68-70. |
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| 作者姓名： | 许俐 王红丽 刘国顺 杨惠娟 |
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| 作者单位： | 河南农业大学烟草学院、国家烟草栽培生理生化研究基地,河南郑州450002 |
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| 基金项目： | 河南省教育厅自然科学基础研究计划项目（2013A180474）. |
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| 摘 要： | 通过对烟草中3个亲环素家族基因（CyPl、CyP2、CyP40）表达模式的研究，结果显示：在不同发育时期中，CyPI在烟草移栽后40、50、60d时表达量较高，CyP2则是在移栽后30、40、60d表达量较高，而CyP40只在移栽后60d表达量较高。在不同组织部位及器官的表达中，3个亲环素家族基因在中部叶和下部叶中表达均较强。在烟草根中主要表达CyPl和CyP40基因，CyP40还在花中表达较高，而CyP2基因的主要表达器官为叶片。
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| 关 键 词： | 烟草 亲环素基因 器官 发育时期 基因表达 |
Expression of Cyclophilin Genes in Different Organs and Leaf of Tobacco at Different Developmental Stages |
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| Institution: | XU Li, WANG Hong- li, LIU Guo- shun, YANG Hui -juan ( College of Tobacco, Henan Agricultural University ; National Research Base for Tobacco Cultivation, Physiology and Biochemistry, Zhengzhou 450002, China) |
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| Abstract: | The expression patterns of three cyclophilin genes （ CyP1, CyP2, CyP40） in tobacco were analyzed. The results showed that： the expression of CyP40 was up regulated at later stage （60 d after transplanting） ; that of CyP1 was up -regulated on the 40th, 50th and 60th day after transplanting; that of CyP2 was up - regulated on the 30th, 40th and 60th day after transplan ting. The expressions of CyP1, CyP2, CyP40 were stronger in the middle leaves and lower leaves. CyP1 and CyP40 had higher ex pression in tobacco roots; CyP40 was also highly expressed in tobacco flowers; while CyP2 was mainly expressed in tobacco leaves. |
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| Keywords: | Tobacco ： Cvclophilin genes ： Organ ： Developmental stae ： Expression of gene |
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