水稻抗除草剂bar基因的遗传研究初报

对水稻恢复系品种"752"与转bar基因抗Basta稳定品系("转嘉59"和"转嘉99")杂交后代的遗传规律进行了连续跟踪研究,结果在F2～F5代中大部分株系的抗感分离比例符合3∶1的分离规律,但也有少数符合1∶1、2∶1、5∶3和5∶1。转基因水稻的抗除草剂特性可以遗传至高世代,通过有性杂交和低世代开始高压淘汰选择,可以在中、高世代获得抗性稳定株系,其中有75.9%的抗性稳定株系自交下一代抗性没有分离,抗性发生了3∶1分离的株系只有24.1%。

Preliminary Study on Inheritance of Herbicide Resistance of bar Transgenic Rice (Oryza sativa)

The inheritance of herbicide resistance of the progenies derived from the crosses of rice restoring line "752" with stable Basta-resistant bar transgenic lines ("Zhuanjia 59" and "Zhuanjia 99") was studied by tracking investigation. The results indicated that the resistant and sensitive segregation pattern of most lines in F-2～F-5 generations accorded with the ratio of 3∶1, however, that of some lines accorded with the ratio of 1∶1,2∶1,5∶3 or 5∶1, the characteristics of herbicide resistance could inherit to the high generations. Rice lines with stable Basta resistance could be obtained in middle and high generations by sexual hybridization and high-pressure screening in lower generations. In addition, in self-crossing generation of stable Basta-resistant lines, 24.1% of lines segregated in resistance and sensitivity (3∶1), while 75.9% of lines did not segregate(1∶0).