| 大活组织培养及再生体系建立的研究 |
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| 引用本文： | 卢晓,刘爽,黄晓非,徐娜,姜长阳.大活组织培养及再生体系建立的研究[J].江西农业学报,2012,24(5):73-75. |
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| 作者姓名： | 卢晓 刘爽 黄晓非 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116081 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目，辽宁师范大学教学改革资助项目 |
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| 摘 要： | 以大活的嫩茎为外植体,进行了大活组织培养及再生体系建立的研究。试验结果表明:MS+6-BA 0.5 mg/L+2,4-D 2.0 mg/L是愈伤组织诱导和继代增殖的理想培养基;在光照条件下MS+ZT 0.6 mg/L+NAA 0.1 mg/L是愈伤组织分化的理想培养基;1/4MS+IAA 0.5 mg/L是不定芽生根和试管苗生根继代增殖的理想培养基。试管苗移栽成活率为92.1%,定植后成活率为96.8%。
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| 关 键 词： | 大活 组织培养 再生体系 建立 |
Study on Tissue Culture and Establishment of Regeneration System in Vitro of Ranunculus sceleratus |
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| LU Xiao
,
LIU Shuang
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HUANG Xiao-fei
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XU Na
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JIANG Chang-yang.Study on Tissue Culture and Establishment of Regeneration System in Vitro of Ranunculus sceleratus[J].Acta Agriculturae Jiangxi,2012,24(5):73-75. |
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| Authors: | LU Xiao LIU Shuang HUANG Xiao-fei XU Na JIANG Chang-yang |
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| Institution: | (College of Life Science,Liaoning Normal University,Dalian 116081,China) |
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| Abstract: | Taking the tender stems of Ranunculus sceleratus as explants,the tissue culture and establishment of regeneration system in vitro of Ranunculus sceleratus were studied.The optimum medium for callus inducement and subculture proliferation was MS+6-BA 0.5 mg/L+2,4-D 2.0 mg/L;under the condition of illumination,the optimum medium for callus differentiation was MS+ZT 0.6 mg/L+NAA 0.1 mg/L;the optimum medium for the rooting of adventitious buds and subculture proliferation of test-tube seedlings was 1/4 MS+ IAA 0.5 mg/L.The transplanting survival rate of the test-tube seedlings was 92.1%,and the stable planting survival rate was 96.8%. |
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| Keywords: | Ranunculus sceleratus Tissue culture Regeneration system Establishment |
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