纤维素降解菌群构建及其降解能力的初步研究

通过对堆肥材料内微生物菌群的连续筛选与驯化,获得高效、稳定纤维素降解菌群,并对其生长特性和降解能力进行了研究,结果表明:降解菌群生长迅速,培养第7天即可达到生长平台期;菌群纤维素酶活性在1～7 d内逐步增加,第7天的酶活性最高;降解菌群对不同纤维素材料的纤维素酶活性差异较大,初始pH值低有利于降解菌群酶活性的提高,其中初始pH值为4.0时的纤维素酶活性最高,为86.54 U。

Preliminary Study on Construction and Degradation Ability of Cellulose -degrading Microbial Flora

The high-efficient and stable cellulose-degrading microbial flora was obtained by the continuous selection and domestication on the microorganism flora in composting materials,then a set of experiments were conducted to study its growth property and cellulose-degrading ability.The results showed that the cellulose-degrading community grew fast and reached stable stage after 7 days’ culture;the cellulose enzyme activity of cellulose-degrading microbial flora increased from the 1st day to the 7th day,and it reached the highest level on the 7th day;the cellulose-degrading microbial flora had greatly different cellulose enzyme activity to different cellulosic materials,and the initial lower pH-value was better for increasing the cellulose enzyme activity of cellulose-degrading microbial flora,when initial pH-value was 4.0,the cellulose enzyme activity was the highest,being 86.54 U.