| 根癌农杆菌介导红颊草莓遗传转化体系的建立 |
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| 引用本文： | 古咸彬,王翡,高志红,乔玉山,章镇,林之林,糜林,李金凤.根癌农杆菌介导红颊草莓遗传转化体系的建立[J].江西农业学报,2012,24(3):11-14. |
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| 作者姓名： | 古咸彬 王翡 高志红 乔玉山 章镇 林之林 糜林 李金凤 |
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| 作者单位： | 1. 南京农业大学园艺学院,江苏南京,210095
2. 江苏丘陵地区镇江农业科学研究所,江苏句容,212400 |
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| 基金项目： | 江苏省农业资源开发局项目(2008KJ-046);2011年国家大学生创新训练计划 |
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| 摘 要： | 以草莓品种红颊的叶片为受体,通过对影响遗传转化的一系列因子:潮霉素选择压、抗生素种类和浓度、农杆菌浓度、侵染时间和共培养时间的优化,获得了较高转化效率的体系。结果表明:菌液浓度为OD600=0.4,侵染时间为6 min,选用250 mg/L的Cb作为抑菌抗生素、5 mg/L的潮霉素作为筛选抗生素,共培养时间3 d是一个高效的转化体系,抗性芽诱导率达到25%。
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| 关 键 词： | 遗传转化 草莓 红颊 |
Establishment of Agrobacterium -mediated Transformation System for ‘ Benihope' Strawberry |
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| GU Xian-bin
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WANG Fei
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GAO Zhi-hong
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QIAO Yu-shan
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ZHANG Zhen
,
LIN Zhi-lin
,
MI Lin
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LI Jin-feng.Establishment of Agrobacterium -mediated Transformation System for ‘ Benihope' Strawberry[J].Acta Agriculturae Jiangxi,2012,24(3):11-14. |
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| Authors: | GU Xian-bin WANG Fei GAO Zhi-hong QIAO Yu-shan ZHANG Zhen LIN Zhi-lin MI Lin LI Jin-feng |
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| Institution: | 1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China; 2.Zhenjiang Institute of Agricultural Sciences in Hilly Region of Jiangsu,Jurong 212400,China) |
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| Abstract: | Taking the leaves of strawberry cultivar ’Benihope’ as experimental acceptor,a higher-efficient genetic transformation system was obtained through the optimization of series of influential factors,such as hygromycin selection stress,type and concentration of antibiotics,agrobacterial concentration,infection time and co-culture time.The results showed that when the agrobacterial concentration was OD600=0.4,the infection time was 6 min,250 mg/L Cb was used as the antibacterial antibiotics,5 mg/L hygromycin was used as the screening antibiotics,and the co-culture time was 3 days,this transformation system was highly efficient,and its inducement rate of resistant shoots reached 25%. |
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| Keywords: | Genetic transformation Strawberry Benihope |
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