| 明水梨叶片不定芽再生体系的建立 |
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| 引用本文： | 李海,乔玉山,李志强,佟兆国,章镇.明水梨叶片不定芽再生体系的建立[J].江西农业学报,2009,21(4). |
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| 作者姓名： | 李海 乔玉山 李志强 佟兆国 章镇 |
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| 作者单位： | 南京农业大学,园艺学院,江苏,南京,210095 |
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| 基金项目： | 江苏省高技术研究项目 |
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| 摘 要： | 以‘明水’梨(Akemizu)叶片为外植体,研究了不同基本培养基,不同浓度噻重氮苯基脲(TDZ)和吲哚丁酸(IBA)的组合,不同暗培养时间以及不同浓度的硝酸银(AgNO3)对梨叶片不定芽再生的影响。试验结果表明,在NN69+TDZ 1.5mg/L+IBA0.2 mg/L+AgNO31.0 mg/L中暗培养25 d后,叶片不定芽再生率最高,达到71.8%。
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| 关 键 词： | 明水梨 不定芽 再生 培养基 |
Regeneration of Adventitious Bud from Leaves of Pyrus pyrifolia Nakai 'Akemizu' |
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| LI Hai,QIAO Yu-shan,LI Zhi-qiang,TONG Zhao-guo,ZHANG Zhen.Regeneration of Adventitious Bud from Leaves of Pyrus pyrifolia Nakai ''Akemizu''[J].Acta Agriculturae Jiangxi,2009,21(4). |
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| Authors: | LI Hai QIAO Yu-shan LI Zhi-qiang TONG Zhao-guo ZHANG Zhen |
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| Institution: | College of Horticulture;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | Using the isolated Leaves of 'Akemizu' pear as the explants,the regeneration system of the adventitious bud from the leaves was studied,and the influences of different basal media,combinations of TDZ and IBA with different concentrations,cultivation time in the dark and AgNO3 concentration on the regeneration of adventitious bud were explored.The results showed that when the leaves were cultivated on the medium NN69+TDZ 1.5 mg/L+IBA 0.2 mg/L+AgNO3 1.0 mg/L for 25 days under dark condition,the regeneration r... |
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| Keywords: | Pyrus pyrifolia Nakai 'Akemizu' Adventitious bud Regeneration Medium |
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