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| 鸡肠上皮细胞体外原代培养研究 | |
| 引用本文： | 杨文平,高峰,许辉堂,施传信,赵国刚,周光宏.鸡肠上皮细胞体外原代培养研究[J].江西农业学报,2007,19(5):113-115,118. |
| 作者姓名： | 杨文平 高峰 许辉堂 施传信 赵国刚 周光宏 |
| 作者单位： | 1. 南京农业大学,动物科技学院,江苏,南京,210095;南京农业大学,农业部农畜产品加工与质量控制重点开放实验室,江苏,南京210095 2. 南京农业大学,动物科技学院,江苏,南京,210095 3. 河南省新乡县农牧局,河南,新乡,453700 4. 南京农业大学,农业部农畜产品加工与质量控制重点开放实验室,江苏,南京210095 |
| 摘 要： | 取1月龄三黄鸡十二指肠的上皮细胞(intestinal epithelial cell,IEC),经分离纯化进行培养。结果表明:胰蛋白酶-EDTA配合使用、短时间消化收获细胞法可获得大量的健全肠绒毛隐窝单位,进一步培养获得的IEC完全可供相关实验研究之用。 |
| 关 键 词： | 鸡 小肠 上皮细胞 体外原代培养 |
| 文章编号： | 1001-8581（2007）05-0113-03 |
| 收稿时间： | 2007-01-11 |
| 修稿时间： | 2007-01-11 |
In Vitro Primary Culture of Intestinal Epithelial Cells of Chicken |
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| YANG Wen-ping,GAO Feng,XU Hui-tang,SHI Chuan-xin,ZHAO Guo-gang,ZHOU Guang-hong.In Vitro Primary Culture of Intestinal Epithelial Cells of Chicken[J].Acta Agriculturae Jiangxi,2007,19(5):113-115,118. | |
| Authors: | YANG Wen-ping GAO Feng XU Hui-tang SHI Chuan-xin ZHAO Guo-gang ZHOU Guang-hong |
| Institution: | 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Agri- cultural and Animal Products Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 3. Xinxiang Agricultural and Husbandry Bureau, Xinxiang 453700, China |
| Abstract: | The intestinal epithelial cells were obtained from one month-old chicken's duodenum for primary culture after being separated and purified.The results showed that abundant healthy intestinal villus was obtained by using the short time digestion technique with a mixture of trypsin and EDTA.After further cultivation,cells can be used for related experiment research. |
| Keywords: | Chicken Small intestine Epithelial cells In vitro primary culture |
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